6HUG
CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with picrotoxin and megabody Mb38.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | Gamma-aminobutyric acid receptor subunit alpha-1 | polymer | 437 | 49852.1 | 2 | UniProt (P08219) Pfam (PF02931) Pfam (PF02932) | Bos taurus (Bovine) | GABA(A) receptor subunit alpha-1 |
| 2 | B, E (B, E) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 473 | 54444.6 | 2 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | GABA(A) receptor subunit beta-3 |
| 3 | C (C) | Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 495 | 56922.1 | 1 | UniProt (P18507) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | GABA(A) receptor subunit gamma-2 |
| 4 | F (G) | Megabody Mb38 | polymer | 539 | 57784.3 | 1 | Lama glama | ||
| 5 | G (F) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1559.4 | 1 | In PDB GlyTouCan (G68668TB) | |||
| 6 | H, J, L (H, J, L) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 7 | I, M (I, M) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 2 | In PDB GlyTouCan (G09724ZC) | |||
| 8 | K (K) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 9 | N, P (A, D) | [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate | non-polymer | 746.6 | 2 | Chemie (PIO) PubChem (16219852) PubChem (52946598) PubChem (49867504) PubChem (53394310) PubChem (73441659) PubChem (124587602) PubChem (131850384) PubChem (132274717) PubChem (99649229) PubChem (9543520) PubChem (136212530) PubChem (136212534) | |||
| 10 | O (C) | (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione | non-polymer | 292.3 | 1 | Chemie (RI5) PubChem (16759291) PubChem (17756773) PubChem (4816) PubChem (176519984) PubChem (23305071) PubChem (23307258) PubChem (23425892) PubChem (177503956) PubChem (24041599) PubChem (24893782) PubChem (31306) PubChem (22524996) PubChem (23307236) PubChem (50919219) PubChem (442292) PubChem (71296878) PubChem (73707459) PubChem (73759784) PubChem (73896911) PubChem (689049) PubChem (1268032) PubChem (88590523) PubChem (88909922) PubChem (5320588) PubChem (5458939) PubChem (98140704) PubChem (98140703) PubChem (5702165) PubChem (6419901) PubChem (6604945) PubChem (6978719) PubChem (6604354) PubChem (98140702) PubChem (98140705) PubChem (117072374) PubChem (7098683) PubChem (122130660) PubChem (122130784) PubChem (124080967) PubChem (12314267) PubChem (12314268) PubChem (126456804) PubChem (170924269) PubChem (172433597) PubChem (129700527) PubChem (131673970) PubChem (145948324) PubChem (145948333) PubChem (155929809) PubChem (162968642) PubChem (162968643) PubChem (164802118) PubChem (171378856) |
Sequence modifications
A, D: 1 - 429 (UniProt: P08219)
C: -38 - 436 (UniProt: P18507)
| PDB | External Database | Details |
|---|---|---|
| Asp -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Lys 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 437 | - | linker |
| Gly 438 | - | linker |
| Ser 439 | - | linker |
| Gly 440 | - | linker |
| Gly 441 | - | linker |
| Ser 442 | - | linker |
| Gly 443 | - | linker |
| Gly 444 | - | linker |
| Ser 445 | - | linker |
| Gly 446 | - | linker |
| Lys 447 | - | linker |
| Thr 448 | - | expression tag |
| Glu 449 | - | expression tag |
| Thr 450 | - | expression tag |
| Ser 451 | - | expression tag |
| Gln 452 | - | expression tag |
| Val 453 | - | expression tag |
| Ala 454 | - | expression tag |
| Pro 455 | - | expression tag |
| Ala 456 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 323299.7 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 5889.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1785.4 | |
| All* | Total formula weight | 330974.4 |
