6HUG

CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with picrotoxin and megabody Mb38.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004888	molecular_function	transmembrane signaling receptor activity
A	0004890	molecular_function	GABA-A receptor activity
A	0005216	molecular_function	monoatomic ion channel activity
A	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
A	0005254	molecular_function	chloride channel activity
A	0005886	cellular_component	plasma membrane
A	0006811	biological_process	monoatomic ion transport
A	0006821	biological_process	chloride transport
A	0007214	biological_process	gamma-aminobutyric acid signaling pathway
A	0008503	molecular_function	benzodiazepine receptor activity
A	0016020	cellular_component	membrane
A	0022851	molecular_function	GABA-gated chloride ion channel activity
A	0030659	cellular_component	cytoplasmic vesicle membrane
A	0031410	cellular_component	cytoplasmic vesicle
A	0032590	cellular_component	dendrite membrane
A	0034220	biological_process	monoatomic ion transmembrane transport
A	0034707	cellular_component	chloride channel complex
A	0043005	cellular_component	neuron projection
A	0045202	cellular_component	synapse
A	0045211	cellular_component	postsynaptic membrane
A	0051932	biological_process	synaptic transmission, GABAergic
A	0060078	biological_process	regulation of postsynaptic membrane potential
A	0098794	cellular_component	postsynapse
A	0099634	cellular_component	postsynaptic specialization membrane
A	1902476	biological_process	chloride transmembrane transport
A	1902495	cellular_component	transmembrane transporter complex
A	1902711	cellular_component	GABA-A receptor complex
A	1904862	biological_process	inhibitory synapse assembly
B	0004888	molecular_function	transmembrane signaling receptor activity
B	0004890	molecular_function	GABA-A receptor activity
B	0005216	molecular_function	monoatomic ion channel activity
B	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
B	0005254	molecular_function	chloride channel activity
B	0005886	cellular_component	plasma membrane
B	0006811	biological_process	monoatomic ion transport
B	0006821	biological_process	chloride transport
B	0007165	biological_process	signal transduction
B	0007214	biological_process	gamma-aminobutyric acid signaling pathway
B	0016020	cellular_component	membrane
B	0022851	molecular_function	GABA-gated chloride ion channel activity
B	0030594	molecular_function	neurotransmitter receptor activity
B	0030659	cellular_component	cytoplasmic vesicle membrane
B	0031410	cellular_component	cytoplasmic vesicle
B	0034220	biological_process	monoatomic ion transmembrane transport
B	0034707	cellular_component	chloride channel complex
B	0042802	molecular_function	identical protein binding
B	0043005	cellular_component	neuron projection
B	0043197	cellular_component	dendritic spine
B	0045202	cellular_component	synapse
B	0045211	cellular_component	postsynaptic membrane
B	0051932	biological_process	synaptic transmission, GABAergic
B	0060021	biological_process	roof of mouth development
B	0071420	biological_process	cellular response to histamine
B	0099634	cellular_component	postsynaptic specialization membrane
B	1902476	biological_process	chloride transmembrane transport
B	1902495	cellular_component	transmembrane transporter complex
B	1902711	cellular_component	GABA-A receptor complex
B	1904862	biological_process	inhibitory synapse assembly
C	0004888	molecular_function	transmembrane signaling receptor activity
C	0004890	molecular_function	GABA-A receptor activity
C	0005216	molecular_function	monoatomic ion channel activity
C	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
C	0006811	biological_process	monoatomic ion transport
C	0006821	biological_process	chloride transport
C	0007214	biological_process	gamma-aminobutyric acid signaling pathway
C	0016020	cellular_component	membrane
C	0034220	biological_process	monoatomic ion transmembrane transport
D	0004888	molecular_function	transmembrane signaling receptor activity
D	0004890	molecular_function	GABA-A receptor activity
D	0005216	molecular_function	monoatomic ion channel activity
D	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
D	0005254	molecular_function	chloride channel activity
D	0005886	cellular_component	plasma membrane
D	0006811	biological_process	monoatomic ion transport
D	0006821	biological_process	chloride transport
D	0007214	biological_process	gamma-aminobutyric acid signaling pathway
D	0008503	molecular_function	benzodiazepine receptor activity
D	0016020	cellular_component	membrane
D	0022851	molecular_function	GABA-gated chloride ion channel activity
D	0030659	cellular_component	cytoplasmic vesicle membrane
D	0031410	cellular_component	cytoplasmic vesicle
D	0032590	cellular_component	dendrite membrane
D	0034220	biological_process	monoatomic ion transmembrane transport
D	0034707	cellular_component	chloride channel complex
D	0043005	cellular_component	neuron projection
D	0045202	cellular_component	synapse
D	0045211	cellular_component	postsynaptic membrane
D	0051932	biological_process	synaptic transmission, GABAergic
D	0060078	biological_process	regulation of postsynaptic membrane potential
D	0098794	cellular_component	postsynapse
D	0099634	cellular_component	postsynaptic specialization membrane
D	1902476	biological_process	chloride transmembrane transport
D	1902495	cellular_component	transmembrane transporter complex
D	1902711	cellular_component	GABA-A receptor complex
D	1904862	biological_process	inhibitory synapse assembly
E	0004888	molecular_function	transmembrane signaling receptor activity
E	0004890	molecular_function	GABA-A receptor activity
E	0005216	molecular_function	monoatomic ion channel activity
E	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
E	0005254	molecular_function	chloride channel activity
E	0005886	cellular_component	plasma membrane
E	0006811	biological_process	monoatomic ion transport
E	0006821	biological_process	chloride transport
E	0007165	biological_process	signal transduction
E	0007214	biological_process	gamma-aminobutyric acid signaling pathway
E	0016020	cellular_component	membrane
E	0022851	molecular_function	GABA-gated chloride ion channel activity
E	0030594	molecular_function	neurotransmitter receptor activity
E	0030659	cellular_component	cytoplasmic vesicle membrane
E	0031410	cellular_component	cytoplasmic vesicle
E	0034220	biological_process	monoatomic ion transmembrane transport
E	0034707	cellular_component	chloride channel complex
E	0042802	molecular_function	identical protein binding
E	0043005	cellular_component	neuron projection
E	0043197	cellular_component	dendritic spine
E	0045202	cellular_component	synapse
E	0045211	cellular_component	postsynaptic membrane
E	0051932	biological_process	synaptic transmission, GABAergic
E	0060021	biological_process	roof of mouth development
E	0071420	biological_process	cellular response to histamine
E	0099634	cellular_component	postsynaptic specialization membrane
E	1902476	biological_process	chloride transmembrane transport
E	1902495	cellular_component	transmembrane transporter complex
E	1902711	cellular_component	GABA-A receptor complex
E	1904862	biological_process	inhibitory synapse assembly

Functional Information from PROSITE/UniProt

site_id	PS00236
Number of Residues	15
Details	NEUROTR_ION_CHANNEL Neurotransmitter-gated ion-channels signature. CmMdLrrYPlDeqnC

Chain	Residue	Details
B	CYS136-CYS150
A	CYS139-CYS153
C	CYS151-CYS165

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	248
Details	TOPO_DOM: Extracellular => ECO:0000305

Chain	Residue	Details
C	GLN1-PHE236
C	ARG284-ALA295
C	LEU434-LEU436
E	LEU268-LYS279
D	ASN275-THR284
D	TYR415-GLN429

---

site_id	SWS_FT_FI2
Number of Residues	83
Details	TRANSMEM: Helical => ECO:0000269|PubMed:30602789, ECO:0007744|PDB:6I53

Chain	Residue	Details
C	THR237-ILE257
C	ALA264-ALA283
C	MET296-PHE320
C	TYR413-TYR433
E	ILE222-ILE242
E	SER247-HIS267
E	ALA280-ASN303
E	ILE423-TYR445

---

site_id	SWS_FT_FI3
Number of Residues	96
Details	TOPO_DOM: Cytoplasmic => ECO:0000305

Chain	Residue	Details
C	ASN258-PRO263
C	VAL321-SER412
E	ASN243-ALA246
E	TYR304-ALA422

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
C	ASN13
C	ASN90

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:29950725, ECO:0000269|PubMed:30602789, ECO:0007744|PDB:6D6T, ECO:0007744|PDB:6I53

Chain	Residue	Details
C	ASN208
B	PHE200
E	ASP95
E	PHE200

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	BINDING: in chain B => ECO:0000269|PubMed:35355020, ECO:0007744|PDB:7QN7

Chain	Residue	Details
B	TYR97
B	SER156
B	THR202
E	TYR97
E	SER156
E	THR202

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	BINDING: in chain A => ECO:0000269|PubMed:35355020, ECO:0007744|PDB:7QN7

Chain	Residue	Details
B	GLU155
E	GLU155

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	BINDING: in chain A => ECO:0000269|PubMed:30140029, ECO:0007744|PDB:6A96

Chain	Residue	Details
B	TYR157
E	TYR157

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:24909990, ECO:0000269|PubMed:35355020, ECO:0007744|PDB:7QN7

Chain	Residue	Details
B	ASN8
E	ASN8

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:24909990, ECO:0000269|PubMed:30602789, ECO:0000269|PubMed:35355020, ECO:0007744|PDB:6I53, ECO:0007744|PDB:7QN7

Chain	Residue	Details
B	ASN80
E	ASN80

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:24909990, ECO:0000269|PubMed:30140029, ECO:0000269|PubMed:30602789, ECO:0000269|PubMed:35355020, ECO:0007744|PDB:6A96, ECO:0007744|PDB:6I53, ECO:0007744|PDB:7QN7

Chain	Residue	Details
B	ASN149
E	ASN149

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