6HJX

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) 7'C pore mutant (L238C) in complex with nanobody 72

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cys-loop ligand-gated ion channel	polymer	306	35244.1	1	UniProt (P0C7B7) Pfam (PF02931) In PDB	Dickeya chrysanthemi (Pectobacterium chrysanthemi)	ELIC
2	B	Cys-loop ligand-gated ion channel	polymer	309	35555.4	1	UniProt (P0C7B7) Pfam (PF02931) In PDB	Dickeya chrysanthemi (Pectobacterium chrysanthemi)	ELIC
3	C	Cys-loop ligand-gated ion channel	polymer	312	35880.8	1	UniProt (P0C7B7) Pfam (PF02931) In PDB	Dickeya chrysanthemi (Pectobacterium chrysanthemi)	ELIC
4	D	Cys-loop ligand-gated ion channel	polymer	310	35726.7	1	UniProt (P0C7B7) Pfam (PF02931) In PDB	Dickeya chrysanthemi (Pectobacterium chrysanthemi)	ELIC
5	E	Cys-loop ligand-gated ion channel	polymer	307	35359.1	1	UniProt (P0C7B7) Pfam (PF02931) In PDB	Dickeya chrysanthemi (Pectobacterium chrysanthemi)	ELIC
6	F, G, H, I, J	nanobody 72	polymer	124	13368.0	5		Lama glama
7	A, B, D, E	HEXAETHYLENE GLYCOL	non-polymer		282.3	4	Chemie (P6G)
8	A	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	1	Chemie (PTY)
9	A, B, C, D, E	DODECYL-BETA-D-MALTOSIDE	non-polymer		510.6	5	Chemie (LMT)
10	C	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
11	C	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
12		water	water		18.0	308	Chemie (HOH)

Sequence modifications

A: 9 - 314 (UniProt: P0C7B7)

PDB	External Database	Details
Gly 164	-	insertion
Cys 238	Leu 237	engineered mutation
Asn 289	Met 288	conflict
Ser 300	Cys 299	engineered mutation
Ser 313	Cys 312	engineered mutation

B: 8 - 314 (UniProt: P0C7B7)

PDB	External Database	Details
Pro 6	-	expression tag
Val 7	-	expression tag
Gly 164	-	insertion
Cys 238	Leu 237	engineered mutation
Asn 289	Met 288	conflict
Ser 300	Cys 299	engineered mutation
Ser 313	Cys 312	engineered mutation

C: 8 - 317 (UniProt: P0C7B7)

PDB	External Database	Details
Pro 6	-	expression tag
Val 7	-	expression tag
Gly 164	-	insertion
Cys 238	Leu 237	engineered mutation
Asn 289	Met 288	conflict
Ser 300	Cys 299	engineered mutation
Ser 313	Cys 312	engineered mutation

D: 9 - 318 (UniProt: P0C7B7)

PDB	External Database	Details
Gly 164	-	insertion
Cys 238	Leu 237	engineered mutation
Asn 289	Met 288	conflict
Ser 300	Cys 299	engineered mutation
Ser 313	Cys 312	engineered mutation

E: 8 - 314 (UniProt: P0C7B7)

PDB	External Database	Details
Gly 164	-	insertion
Cys 238	Leu 237	engineered mutation
Asn 289	Met 288	conflict
Ser 300	Cys 299	engineered mutation
Ser 313	Cys 312	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	244605.9
Non-Polymers*	Number of molecules	12
	Total formula weight	4634.7
All*	Total formula weight	249240.6

*Water molecules are not included.