6H3C

Cryo-EM structure of the BRISC complex bound to SHMT2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, F)	BRISC complex subunit Abraxas 2	polymer	434	49256.2	2	UniProt (Q15018) Pfam (PF21125)	Homo sapiens (Human)	Abraxas brother protein 1,Protein FAM175B
2	B, F (B, G)	Lys-63-specific deubiquitinase BRCC36	polymer	335	38417.4	2	UniProt (P46736) Pfam (PF01398) Pfam (PF18110)	Homo sapiens (Human)	BRCA1-A complex subunit BRCC36,BRCA1/BRCA2-containing complex subunit 3,BRCA1/BRCA2-containing complex subunit 36,BRISC complex subunit BRCC36
3	C, G (C, H)	BRISC and BRCA1-A complex member 2	polymer	402	45890.9	2	UniProt (Q9NXR7) Pfam (PF06113)	Homo sapiens (Human)	BRCA1-A complex subunit BRE,BRCA1/BRCA2-containing complex subunit 45,Brain and reproductive organ-expressed protein
4	D, H (D, I)	BRISC and BRCA1-A complex member 1	polymer	352	39263.8	2	UniProt (Q9NWV8)	Homo sapiens (Human)	Mediator of RAP80 interactions and targeting subunit of 40 kDa,New component of the BRCA1-A complex
5	I, J (E, J)	Serine hydroxymethyltransferase, mitochondrial	polymer	507	56144.7	2	UniProt (P34897) Pfam (PF00464)	Homo sapiens (Human)	SHMT,Glycine hydroxymethyltransferase,Serine methylase
6	K, L (B, G)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	M, N (B, G)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, F: 1 - 415 (UniProt: Q15018)

PDB	External Database	Details
Met -18	-	initiating methionine
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

B, G: 1 - 316 (UniProt: P46736)

PDB	External Database	Details
Met -18	-	initiating methionine
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

C, H: 1 - 383 (UniProt: Q9NXR7)

PDB	External Database	Details
Met -18	-	initiating methionine
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

D, I: 1 - 329 (UniProt: Q9NWV8)

PDB	External Database	Details
Met -22	-	initiating methionine
Ala -21	-	expression tag
Ser -20	-	expression tag
Trp -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Pro -16	-	expression tag
Gln -15	-	expression tag
Phe -14	-	expression tag
Glu -13	-	expression tag
Lys -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

E, J: -4 - 483 (UniProt: P34897)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Gly -11	-	expression tag
Leu -10	-	expression tag
Val -9	-	expression tag
Pro -8	-	expression tag
Arg -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Thr 264	Ala 285	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	457946.3
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	458077.1

*Water molecules are not included.