6H3C

Cryo-EM structure of the BRISC complex bound to SHMT2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000278	biological_process	mitotic cell cycle
A	0000922	cellular_component	spindle pole
A	0002931	biological_process	response to ischemia
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005813	cellular_component	centrosome
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0007059	biological_process	chromosome segregation
A	0008017	molecular_function	microtubule binding
A	0008608	biological_process	attachment of spindle microtubules to kinetochore
A	0030496	cellular_component	midbody
A	0031593	molecular_function	polyubiquitin modification-dependent protein binding
A	0031616	cellular_component	spindle pole centrosome
A	0035869	cellular_component	ciliary transition zone
A	0036064	cellular_component	ciliary basal body
A	0036449	cellular_component	microtubule minus-end
A	0051301	biological_process	cell division
A	0070536	biological_process	protein K63-linked deubiquitination
A	0070552	cellular_component	BRISC complex
A	0090307	biological_process	mitotic spindle assembly
B	0000151	cellular_component	ubiquitin ligase complex
B	0000152	cellular_component	nuclear ubiquitin ligase complex
B	0000922	cellular_component	spindle pole
B	0004843	molecular_function	cysteine-type deubiquitinase activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006281	biological_process	DNA repair
B	0006282	biological_process	regulation of DNA repair
B	0006302	biological_process	double-strand break repair
B	0006325	biological_process	chromatin organization
B	0006508	biological_process	proteolysis
B	0006974	biological_process	DNA damage response
B	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
B	0008233	molecular_function	peptidase activity
B	0008237	molecular_function	metallopeptidase activity
B	0010165	biological_process	response to X-ray
B	0010212	biological_process	response to ionizing radiation
B	0016579	biological_process	protein deubiquitination
B	0016787	molecular_function	hydrolase activity
B	0030234	molecular_function	enzyme regulator activity
B	0031593	molecular_function	polyubiquitin modification-dependent protein binding
B	0044818	biological_process	mitotic G2/M transition checkpoint
B	0045739	biological_process	positive regulation of DNA repair
B	0046872	molecular_function	metal ion binding
B	0051301	biological_process	cell division
B	0061578	molecular_function	K63-linked deubiquitinase activity
B	0070531	cellular_component	BRCA1-A complex
B	0070536	biological_process	protein K63-linked deubiquitination
B	0070552	cellular_component	BRISC complex
B	0071479	biological_process	cellular response to ionizing radiation
B	0140492	molecular_function	metal-dependent deubiquitinase activity
B	0140861	biological_process	DNA repair-dependent chromatin remodeling
B	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
B	2000001	biological_process	regulation of DNA damage checkpoint
C	0000152	cellular_component	nuclear ubiquitin ligase complex
C	0000268	molecular_function	peroxisome targeting sequence binding
C	0005164	molecular_function	tumor necrosis factor receptor binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006281	biological_process	DNA repair
C	0006282	biological_process	regulation of DNA repair
C	0006302	biological_process	double-strand break repair
C	0006325	biological_process	chromatin organization
C	0006915	biological_process	apoptotic process
C	0006974	biological_process	DNA damage response
C	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
C	0007165	biological_process	signal transduction
C	0010212	biological_process	response to ionizing radiation
C	0031593	molecular_function	polyubiquitin modification-dependent protein binding
C	0043066	biological_process	negative regulation of apoptotic process
C	0044818	biological_process	mitotic G2/M transition checkpoint
C	0045739	biological_process	positive regulation of DNA repair
C	0051301	biological_process	cell division
C	0070531	cellular_component	BRCA1-A complex
C	0070536	biological_process	protein K63-linked deubiquitination
C	0070552	cellular_component	BRISC complex
C	0071479	biological_process	cellular response to ionizing radiation
C	2000001	biological_process	regulation of DNA damage checkpoint
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006281	biological_process	DNA repair
D	0006282	biological_process	regulation of DNA repair
D	0006302	biological_process	double-strand break repair
D	0006325	biological_process	chromatin organization
D	0006974	biological_process	DNA damage response
D	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
D	0010212	biological_process	response to ionizing radiation
D	0016604	cellular_component	nuclear body
D	0042802	molecular_function	identical protein binding
D	0044818	biological_process	mitotic G2/M transition checkpoint
D	0045739	biological_process	positive regulation of DNA repair
D	0051301	biological_process	cell division
D	0070531	cellular_component	BRCA1-A complex
D	0070552	cellular_component	BRISC complex
D	0071425	biological_process	hematopoietic stem cell proliferation
D	0140861	biological_process	DNA repair-dependent chromatin remodeling
E	0002082	biological_process	regulation of oxidative phosphorylation
E	0003682	molecular_function	chromatin binding
E	0004372	molecular_function	glycine hydroxymethyltransferase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005743	cellular_component	mitochondrial inner membrane
E	0005759	cellular_component	mitochondrial matrix
E	0006231	biological_process	dTMP biosynthetic process
E	0006417	biological_process	regulation of translation
E	0006544	biological_process	glycine metabolic process
E	0006545	biological_process	glycine biosynthetic process
E	0006563	biological_process	L-serine metabolic process
E	0006564	biological_process	L-serine biosynthetic process
E	0006730	biological_process	one-carbon metabolic process
E	0008284	biological_process	positive regulation of cell population proliferation
E	0008732	molecular_function	L-allo-threonine aldolase activity
E	0015630	cellular_component	microtubule cytoskeleton
E	0015943	biological_process	formate biosynthetic process
E	0016597	molecular_function	amino acid binding
E	0016740	molecular_function	transferase activity
E	0019264	biological_process	glycine biosynthetic process from serine
E	0030170	molecular_function	pyridoxal phosphate binding
E	0034340	biological_process	response to type I interferon
E	0035999	biological_process	tetrahydrofolate interconversion
E	0042645	cellular_component	mitochondrial nucleoid
E	0042802	molecular_function	identical protein binding
E	0046653	biological_process	tetrahydrofolate metabolic process
E	0051262	biological_process	protein tetramerization
E	0051289	biological_process	protein homotetramerization
E	0070013	cellular_component	intracellular organelle lumen
E	0070062	cellular_component	extracellular exosome
E	0070129	biological_process	regulation of mitochondrial translation
E	0070536	biological_process	protein K63-linked deubiquitination
E	0070552	cellular_component	BRISC complex
E	0120567	molecular_function	hydroxytrimethyllysine aldolase activity
E	1903715	biological_process	regulation of aerobic respiration
F	0000278	biological_process	mitotic cell cycle
F	0000922	cellular_component	spindle pole
F	0002931	biological_process	response to ischemia
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005813	cellular_component	centrosome
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005874	cellular_component	microtubule
F	0007059	biological_process	chromosome segregation
F	0008017	molecular_function	microtubule binding
F	0008608	biological_process	attachment of spindle microtubules to kinetochore
F	0030496	cellular_component	midbody
F	0031593	molecular_function	polyubiquitin modification-dependent protein binding
F	0031616	cellular_component	spindle pole centrosome
F	0035869	cellular_component	ciliary transition zone
F	0036064	cellular_component	ciliary basal body
F	0036449	cellular_component	microtubule minus-end
F	0051301	biological_process	cell division
F	0070536	biological_process	protein K63-linked deubiquitination
F	0070552	cellular_component	BRISC complex
F	0090307	biological_process	mitotic spindle assembly
G	0000151	cellular_component	ubiquitin ligase complex
G	0000152	cellular_component	nuclear ubiquitin ligase complex
G	0000922	cellular_component	spindle pole
G	0004843	molecular_function	cysteine-type deubiquitinase activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006281	biological_process	DNA repair
G	0006282	biological_process	regulation of DNA repair
G	0006302	biological_process	double-strand break repair
G	0006325	biological_process	chromatin organization
G	0006508	biological_process	proteolysis
G	0006974	biological_process	DNA damage response
G	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
G	0008233	molecular_function	peptidase activity
G	0008237	molecular_function	metallopeptidase activity
G	0010165	biological_process	response to X-ray
G	0010212	biological_process	response to ionizing radiation
G	0016579	biological_process	protein deubiquitination
G	0016787	molecular_function	hydrolase activity
G	0030234	molecular_function	enzyme regulator activity
G	0031593	molecular_function	polyubiquitin modification-dependent protein binding
G	0044818	biological_process	mitotic G2/M transition checkpoint
G	0045739	biological_process	positive regulation of DNA repair
G	0046872	molecular_function	metal ion binding
G	0051301	biological_process	cell division
G	0061578	molecular_function	K63-linked deubiquitinase activity
G	0070531	cellular_component	BRCA1-A complex
G	0070536	biological_process	protein K63-linked deubiquitination
G	0070552	cellular_component	BRISC complex
G	0071479	biological_process	cellular response to ionizing radiation
G	0140492	molecular_function	metal-dependent deubiquitinase activity
G	0140861	biological_process	DNA repair-dependent chromatin remodeling
G	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
G	2000001	biological_process	regulation of DNA damage checkpoint
H	0000152	cellular_component	nuclear ubiquitin ligase complex
H	0000268	molecular_function	peroxisome targeting sequence binding
H	0005164	molecular_function	tumor necrosis factor receptor binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006281	biological_process	DNA repair
H	0006282	biological_process	regulation of DNA repair
H	0006302	biological_process	double-strand break repair
H	0006325	biological_process	chromatin organization
H	0006915	biological_process	apoptotic process
H	0006974	biological_process	DNA damage response
H	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
H	0007165	biological_process	signal transduction
H	0010212	biological_process	response to ionizing radiation
H	0031593	molecular_function	polyubiquitin modification-dependent protein binding
H	0043066	biological_process	negative regulation of apoptotic process
H	0044818	biological_process	mitotic G2/M transition checkpoint
H	0045739	biological_process	positive regulation of DNA repair
H	0051301	biological_process	cell division
H	0070531	cellular_component	BRCA1-A complex
H	0070536	biological_process	protein K63-linked deubiquitination
H	0070552	cellular_component	BRISC complex
H	0071479	biological_process	cellular response to ionizing radiation
H	2000001	biological_process	regulation of DNA damage checkpoint
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006281	biological_process	DNA repair
I	0006282	biological_process	regulation of DNA repair
I	0006302	biological_process	double-strand break repair
I	0006325	biological_process	chromatin organization
I	0006974	biological_process	DNA damage response
I	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
I	0010212	biological_process	response to ionizing radiation
I	0016604	cellular_component	nuclear body
I	0042802	molecular_function	identical protein binding
I	0044818	biological_process	mitotic G2/M transition checkpoint
I	0045739	biological_process	positive regulation of DNA repair
I	0051301	biological_process	cell division
I	0070531	cellular_component	BRCA1-A complex
I	0070552	cellular_component	BRISC complex
I	0071425	biological_process	hematopoietic stem cell proliferation
I	0140861	biological_process	DNA repair-dependent chromatin remodeling
J	0002082	biological_process	regulation of oxidative phosphorylation
J	0003682	molecular_function	chromatin binding
J	0004372	molecular_function	glycine hydroxymethyltransferase activity
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005739	cellular_component	mitochondrion
J	0005743	cellular_component	mitochondrial inner membrane
J	0005759	cellular_component	mitochondrial matrix
J	0006231	biological_process	dTMP biosynthetic process
J	0006417	biological_process	regulation of translation
J	0006544	biological_process	glycine metabolic process
J	0006545	biological_process	glycine biosynthetic process
J	0006563	biological_process	L-serine metabolic process
J	0006564	biological_process	L-serine biosynthetic process
J	0006730	biological_process	one-carbon metabolic process
J	0008284	biological_process	positive regulation of cell population proliferation
J	0008732	molecular_function	L-allo-threonine aldolase activity
J	0015630	cellular_component	microtubule cytoskeleton
J	0015943	biological_process	formate biosynthetic process
J	0016597	molecular_function	amino acid binding
J	0016740	molecular_function	transferase activity
J	0019264	biological_process	glycine biosynthetic process from serine
J	0030170	molecular_function	pyridoxal phosphate binding
J	0034340	biological_process	response to type I interferon
J	0035999	biological_process	tetrahydrofolate interconversion
J	0042645	cellular_component	mitochondrial nucleoid
J	0042802	molecular_function	identical protein binding
J	0046653	biological_process	tetrahydrofolate metabolic process
J	0051262	biological_process	protein tetramerization
J	0051289	biological_process	protein homotetramerization
J	0070013	cellular_component	intracellular organelle lumen
J	0070062	cellular_component	extracellular exosome
J	0070129	biological_process	regulation of mitochondrial translation
J	0070536	biological_process	protein K63-linked deubiquitination
J	0070552	cellular_component	BRISC complex
J	0120567	molecular_function	hydroxytrimethyllysine aldolase activity
J	1903715	biological_process	regulation of aerobic respiration

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN B 401

Chain	Residue
B	HIS122
B	HIS124
B	ASP135
B	HOH1000

---

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN G 401

Chain	Residue
G	HIS122
G	HIS124
G	ASP135
G	HOH1000

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	292
Details	Domain: {"description":"MPN","evidences":[{"source":"PROSITE-ProRule","id":"PRU01182","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	14
Details	Region: {"description":"Important for interaction with SHMT2","evidences":[{"source":"PubMed","id":"24075985","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	42
Details	Region: {"description":"Important for interaction with BBRC36 and other subunits of the BRISC complex","evidences":[{"source":"PubMed","id":"26344097","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	26
Details	Motif: {"description":"JAMM motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01182","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01182","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	234
Details	Region: {"description":"UEV-like 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	178
Details	Region: {"description":"UEV-like 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22223895","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	406
Details	Region: {"description":"VWFA-like"}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	14
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"PubMed","id":"29180469","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---