6GO6

TdT chimera (Loop1 of pol mu) - ternary complex with downstream dsDNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA nucleotidylexotransferase,DNA-directed DNA/RNA polymerase mu,DNA nucleotidylexotransferase	polymer	401	45849.0	1	UniProt (P09838) UniProt (Q9JIW4) Pfam (PF14716) Pfam (PF10391) Pfam (PF01909) Pfam (PF14791)	Mus musculus (Mouse)	Terminal addition enzyme,Terminal deoxynucleotidyltransferase,Terminal transferase,Pol Mu,Terminal transferase,Terminal addition enzyme,Terminal deoxynucleotidyltransferase,Terminal transferase
2	B, C (G, F)	DNA (5'-D(*TP*TP*TP*TP*TP*GP*GP*C)-3')	polymer	8	2423.6	2		synthetic construct
3	D (E)	DNA (5'-D(*AP*AP*AP*AP*AP*C)-3')	polymer	6	1810.3	1		synthetic construct
4	E (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	F (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	G (A)	2',3'-DIDEOXYCYTIDINE 5'-TRIPHOSPHATE	non-polymer		451.2	1	Chemie (DCT)
7	H, I, J, K (A, G, F, E)	water	water		18.0	329	Chemie (HOH)

Sequence modifications

A: 132 - 377 (UniProt: P09838)

PDB	External Database	Details
Met 111	-	initiating methionine
Gly 112	-	expression tag
Ser 113	-	expression tag
Ser 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
Ser 121	-	expression tag
Ser 122	-	expression tag
Gly 123	-	expression tag
Leu 124	-	expression tag
Val 125	-	expression tag
Pro 126	-	expression tag
Arg 127	-	expression tag
Gly 128	-	expression tag
Ser 129	-	expression tag
His 130	-	expression tag
Met 131	-	expression tag

A: 378 - 407 (UniProt: Q9JIW4)

PDB	External Database	Details
Val 401	Ala 386	conflict

A: 408 - 511 (UniProt: P09838)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52506.4
Non-Polymers*	Number of molecules	3
	Total formula weight	498.5
All*	Total formula weight	53004.9

*Water molecules are not included.