6GO6

TdT chimera (Loop1 of pol mu) - ternary complex with downstream dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9724
Spacegroup name	P 21 21 2
Unit cell lengths	86.420, 189.830, 46.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.460 - 2.090
R-factor	0.204
Rwork	0.202
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jms
RMSD bond length	0.010
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.460	2.220
High resolution limit [Å]	2.090	2.090
Rmerge	0.057	1.070
Number of reflections	45742	6958
<I/σ(I)>	15.79
Completeness [%]	98.9	94.1
Redundancy	5.4
CC(1/2)	0.999	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	19-25% PEG 4000, 100-400 mM lithium sulfate, 100 mM tris