6GO5

TdT chimera (Loop1 of pol mu) - Ternary complex with 1-nt gapped DNA substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA nucleotidylexotransferase,DNA-directed DNA/RNA polymerase mu,DNA nucleotidylexotransferase	polymer	401	45849.0	2	UniProt (P09838) UniProt (Q9JIW4) Pfam (PF14716) Pfam (PF10391) Pfam (PF01909) Pfam (PF14791)	Mus musculus (Mouse)	Terminal addition enzyme,Terminal deoxynucleotidyltransferase,Terminal transferase,Pol Mu,Terminal transferase,Terminal addition enzyme,Terminal deoxynucleotidyltransferase,Terminal transferase
2	C, G (H, N)	DNA (5'-D(*CP*GP*CP*TP*GP*GP*CP*AP*AP*AP*CP*A)-3')	polymer	12	3656.4	2		synthetic construct
3	D, F (F, L)	DNA (5'-D(*TP*GP*TP*TP*TP*G)-3')	polymer	6	1830.2	2		synthetic construct
4	E, H (G, M)	DNA (5'-D(*AP*CP*AP*GP*CP*G)-3')	polymer	6	1818.2	2		synthetic construct
5	I, M (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
6	J, L (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	K, N (A, B)	2'-deoxy-5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine	non-polymer		465.2	2	Chemie (XC5)
8	O, P, Q, R, S... (A, B, H, F, G...)	water	water		18.0	327	Chemie (HOH)

Sequence modifications

A, B: 132 - 377 (UniProt: P09838)

PDB	External Database	Details
Met 111	-	initiating methionine
Gly 112	-	expression tag
Ser 113	-	expression tag
Ser 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
Ser 121	-	expression tag
Ser 122	-	expression tag
Gly 123	-	expression tag
Leu 124	-	expression tag
Val 125	-	expression tag
Pro 126	-	expression tag
Arg 127	-	expression tag
Gly 128	-	expression tag
Ser 129	-	expression tag
His 130	-	expression tag
Met 131	-	expression tag

A, B: 378 - 407 (UniProt: Q9JIW4)

PDB	External Database	Details
Val 401	Ala 386	conflict

A, B: 408 - 511 (UniProt: P09838)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	106307.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1025.0
All*	Total formula weight	107332.7

*Water molecules are not included.