6GFF

Structure of GARP (LRRC32) in complex with latent TGF-beta1 and MHG-8 Fab

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	Transforming growth factor beta-1	polymer	249	28531.5	4	UniProt (P01137) Pfam (PF00688)	Homo sapiens (Human)	TGF-beta-1
2	B, D, F, H (B, D, F, H)	Transforming growth factor beta-1	polymer	112	12809.8	4	UniProt (P01137) Pfam (PF00019)	Homo sapiens (Human)	TGF-beta-1
3	I, J (I, J)	Leucine-rich repeat-containing protein 32	polymer	618	67235.6	2	UniProt (Q14392) Pfam (PF01462) Pfam (PF13855)	Homo sapiens (Human)	Garpin,Glycoprotein A repetitions predominant,GARP
4	K, M (K, M)	MHG-8 Fab light chain	polymer	212	23376.8	2		Mus musculus (Mouse)
5	L, N (L, N)	MHG-8 Fab heavy chain	polymer	221	23832.6	2		Mus musculus
6	O, P, Q, S, T (O, P, Q, S, T)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	5	In PDB GlyTouCan (G15407YE)
7	R (R)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
8	U (U)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G22573RC)

Sequence modifications

I, J: 20 - 628 (UniProt: Q14392)

PDB	External Database	Details
Glu 629	-	expression tag
Ala 630	-	expression tag
Ala 631	-	expression tag
Glu 632	-	expression tag
Asn 633	-	expression tag
Leu 634	-	expression tag
Tyr 635	-	expression tag
Phe 636	-	expression tag
Gln 637	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	394255.1
Branched	Number of molecules	7
	Total formula weight	4105.8
All*	Total formula weight	398360.9

*Water molecules are not included.