6F4Q

Human JMJD5 (Q275C) in complex with Mn(II), NOG and RPS6-A138C (129-144) (complex-2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	JmjC domain-containing protein 5	polymer	255	29449.1	1	UniProt (Q8N371) Pfam (PF13621) In PDB	Homo sapiens (Human)	LYSINE-SPECIFIC DEMETHYLASE 8, JUMONJI DOMAIN-CONTAINING PROTEIN 5
2	B	40S ribosomal protein S6	polymer	16	1942.5	1	UniProt (P62753) In PDB	Homo sapiens	Phosphoprotein NP33,Small ribosomal subunit protein eS6
3	A	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
4	A	N-OXALYLGLYCINE	non-polymer		147.1	1	Chemie (OGA)
5	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
6	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7		water	water		18.0	361	Chemie (HOH)

Sequence modifications

A: 183 - 416 (UniProt: Q8N371)

PDB	External Database	Details
His 162	-	expression tag
His 163	-	expression tag
His 164	-	expression tag
His 165	-	expression tag
His 166	-	expression tag
His 167	-	expression tag
Ser 168	-	expression tag
Ser 169	-	expression tag
Gly 170	-	expression tag
Val 171	-	expression tag
Asp 172	-	expression tag
Leu 173	-	expression tag
Gly 174	-	expression tag
Thr 175	-	expression tag
Glu 176	-	expression tag
Asn 177	-	expression tag
Leu 178	-	expression tag
Tyr 179	-	expression tag
Phe 180	-	expression tag
Gln 181	-	expression tag
Ser 182	-	expression tag
Ala 217	Cys 255	engineered mutation
Ala 232	Cys 270	engineered mutation
Cys 275	Gln 313	engineered mutation
Ala 295	Cys 333	engineered mutation
Ala 384	Cys 422	engineered mutation

B: 129 - 144 (UniProt: P62753)

PDB	External Database	Details
Cys 138	Ala 138	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	31391.6
Non-Polymers*	Number of molecules	5
	Total formula weight	508.4
All*	Total formula weight	31899.9

*Water molecules are not included.