6F4Q

Human JMJD5 (Q275C) in complex with Mn(II), NOG and RPS6-A138C (129-144) (complex-2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	49.406, 65.073, 77.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.907 - 1.120
R-factor	0.1399
Rwork	0.139
R-free	0.15330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f4p
RMSD bond length	0.010
RMSD bond angle	1.162
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.910	1.160
High resolution limit [Å]	1.120	1.120
Rmerge	0.067	1.100
Rpim	0.022	0.411
Number of reflections	95694	9219
<I/σ(I)>	32.9
Completeness [%]	98.7	96.3
Redundancy	11.1	9.9
CC(1/2)	0.999	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	0.1M Bis-Tris pH 6.0, 19.3 % PEG3350, 0.002 M MnCl2, 300 nl sitting drops (sample:well, 1:1 ratio)