6F1T

Cryo-EM structure of two dynein tail domains bound to dynactin and BICDR1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	ARP1 actin related protein 1 homolog A	polymer	376	42670.7	8	UniProt (F2Z5G5) Pfam (PF00022)	Sus scrofa (Pig)
2	H (H)	Actin, cytoplasmic 1	polymer	375	41782.7	1	UniProt (Q6QAQ1) Pfam (PF00022)	Sus scrofa (Pig)	Beta-actin
3	J (J)	Actin related protein 10 homolog	polymer	390	43203.3	1	UniProt (I3LHK5) Pfam (PF00022)	Sus scrofa (Pig)
4	K (K)	Capping protein (Actin filament) muscle Z-line, alpha 1	polymer	286	33059.8	1	UniProt (A0PFK5) Pfam (PF01267)	Sus scrofa (Pig)	F-actin capping protein alpha 1 subunit,F-actin capping protein subunit alpha 1
5	L (L)	F-actin capping protein beta subunit	polymer	272	30669.8	1	UniProt (A9XFX6) Pfam (PF01115) UniProt (by SIFTS) (A0PFK7)	Sus scrofa (Pig)	F-actin-capping protein subunit beta,F-actin-capping protein subunit beta isoform 1
6	M (M)	Dynactin Subunit 2	polymer	589	50144.9	1		Sus scrofa (Pig)
7	N (N)	Dynactin Subunit 2	polymer	618	52613.0	1		Sus scrofa (Pig)
8	O, P (O, P)	Dynactin Subunit 3	polymer	65	5549.8	2		Sus scrofa (Pig)
9	Q, R (Q, R)	Dynactin Subunit 2	polymer	87	7422.1	2		Sus scrofa (Pig)
10	S (U)	Dynactin 6	polymer	190	20703.9	1	UniProt (D0G6S1)	Sus scrofa (Pig)
11	T (V)	Dynactin subunit 5	polymer	182	20150.5	1	UniProt (A0A286ZK88) Pfam (PF21711)	Sus scrofa (Pig)
12	U (X)	BICD family-like cargo adapter 1,BICD family-like cargo adapter 1,BICD family-like cargo adapter 1,BICDR-1	polymer	389	42423.0	1	UniProt (A0JNT9)	Mus musculus (Mouse)	Bicaudal D-related protein 1,BICDR-1,Coiled-coil domain-containing protein 64A,Bicaudal D-related protein 1,BICDR-1,Coiled-coil domain-containing protein 64A,Bicaudal D-related protein 1,BICDR-1,Coiled-coil domain-containing protein 64A
13	V (Y)	Dynactin Subunit 4	polymer	263	22400.5	1		Sus scrofa (Pig)
14	W (Z)	Dynactin Subunit 1	polymer	52	4443.5	1		Sus scrofa (Pig)
15	X (a)	Dynactin subunit 2	polymer	66	7377.0	1	UniProt (F1SKF9) UniProt (by SIFTS) (A0A5G2QD80)	Sus scrofa (Pig)
16	Y (b)	Dynactin subunit 2	polymer	89	10000.9	1	UniProt (F1SKF9) UniProt (by SIFTS) (A0A5G2QD80)	Sus scrofa (Pig)
17	Z (c)	Dynactin subunit 2	polymer	50	5582.0	1	UniProt (F1SKF9) UniProt (by SIFTS) (A0A5G2QD80)	Sus scrofa (Pig)
18	AA (d)	Dynactin subunit 2	polymer	26	2880.1	1	UniProt (F1SKF9) UniProt (by SIFTS) (A0A5G2QD80)	Sus scrofa (Pig)
19	BA, CA, JA, KA (e, f, m, n)	Cytoplasmic dynein 1 heavy chain 1,Cytoplasmic dynein 1 heavy chain 1,Cytoplasmic dynein 1 heavy chain 1,Dynein Heavy Chain,Cytoplasmic dynein 1 heavy chain 1,Cytoplasmic dynein 1 heavy chain 1,Cytoplasmic dynein 1 heavy chain 1,Cytoplasmic dynein 1 heavy chain 1,Dynein Heavy Chain,Cytoplasmic dynein 1 heavy chain 1,Cytoplasmic dynein 1 heavy chain 1	polymer	1053	119509.4	4	UniProt (Q14204) Pfam (PF08385)	Homo sapiens (Human)	Cytoplasmic dynein heavy chain 1,Dynein heavy chain,cytosolic,Cytoplasmic dynein heavy chain 1,Dynein heavy chain,cytosolic,Cytoplasmic dynein heavy chain 1,Dynein heavy chain,cytosolic,Cytoplasmic dynein heavy chain 1,Dynein heavy chain,cytosolic,Cytoplasmic dynein heavy chain 1,Dynein heavy chain,cytosolic,Cytoplasmic dynein heavy chain 1,Dynein heavy chain,cytosolic,Cytoplasmic dynein heavy chain 1,Dynein heavy chain,cytosolic,Cytoplasmic dynein heavy chain 1,Dynein heavy chain,cytosolic,Cytoplasmic dynein heavy chain 1,Dynein heavy chain,cytosolic
20	DA, EA, LA, MA (g, h, o, p)	Cytoplasmic dynein 1 intermediate chain 2	polymer	612	68442.1	4	UniProt (Q13409) Pfam (PF11540) Pfam (PF00400)	Homo sapiens (Human)	Cytoplasmic dynein intermediate chain 2,Dynein intermediate chain 2,cytosolic,DH IC-2
21	FA, GA, NA, OA (i, j, q, r)	Cytoplasmic dynein 1 light intermediate chain 2	polymer	492	54173.2	4	UniProt (O43237) Pfam (PF05783)	Homo sapiens (Human)	Dynein light intermediate chain 2,cytosolic,LIC-2,LIC53/55
22	HA, IA, PA, QA (k, l, s, t)	Dynein light chain roadblock-type 1	polymer	96	10934.6	4	UniProt (Q9NP97) Pfam (PF03259)	Homo sapiens (Human)	Bithoraxoid-like protein,BLP,Dynein light chain 2A,cytoplasmic,Dynein-associated protein Km23,Roadblock domain-containing protein 1
23	RA (x)	BICD family-like cargo adapter 1,BICD family-like cargo adapter 1,BICD family-like cargo adapter 1	polymer	392	42678.4	1	UniProt (A0JNT9)	Mus musculus (Mouse)	Bicaudal D-related protein 1,BICDR-1,Coiled-coil domain-containing protein 64A,Bicaudal D-related protein 1,BICDR-1,Coiled-coil domain-containing protein 64A,Bicaudal D-related protein 1,BICDR-1,Coiled-coil domain-containing protein 64A
24	SA (z)	Dynactin Subunit 1	polymer	53	4528.6	1		Sus scrofa (Pig)
25	BB, CB, TA, UA, VA... (I, J, A, B, C...)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	9	Chemie (ADP)
26	AB (H)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)

Sequence modifications

X: 1 - 325 (UniProt: A0JNT9)
X: 326 - 389 (PDB: 6F1T)
a: 1 - 66 (UniProt: F1SKF9)

PDB	External Database	Details
Leu 37	Gln 37	conflict

b: 1 - 89 (UniProt: F1SKF9)

PDB	External Database	Details
Leu 37	Gln 37	conflict

e, f, m, n: 1 - 983 (UniProt: Q14204)
e, f, m, n: 984 - 1032 (PDB: 6F1T)
e, f, m, n: 1033 - 1053 (PDB: 6F1T)
g, h, o, p: 1 - 612 (UniProt: Q13409)

PDB	External Database	Details
Ser 484	Thr 484	conflict
Gly 499	Asp 499	conflict

x: 1 - 325 (UniProt: A0JNT9)
x: 326 - 392 (PDB: 6F1T)

Contents of the asymmetric unit

Polymers	Number of chains	45
	Total formula weight	1814188.2
Non-Polymers*	Number of molecules	10
	Total formula weight	4352.0
All*	Total formula weight	1818540.2

*Water molecules are not included.