Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6F1T

Cryo-EM structure of two dynein tail domains bound to dynactin and BICDR1

Functional Information from GO Data

Chain	GOid	namespace	contents
H	0000166	molecular_function	nucleotide binding
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005856	cellular_component	cytoskeleton
H	0005884	cellular_component	actin filament
H	0005886	cellular_component	plasma membrane
H	0005925	cellular_component	focal adhesion
H	0007409	biological_process	axonogenesis
H	0015629	cellular_component	actin cytoskeleton
H	0016020	cellular_component	membrane
H	0016787	molecular_function	hydrolase activity
H	0019901	molecular_function	protein kinase binding
H	0030424	cellular_component	axon
H	0032991	cellular_component	protein-containing complex
H	0035267	cellular_component	NuA4 histone acetyltransferase complex
H	0045202	cellular_component	synapse
H	0048870	biological_process	cell motility
H	0097433	cellular_component	dense body
H	0098973	molecular_function	structural constituent of postsynaptic actin cytoskeleton
H	0098974	biological_process	postsynaptic actin cytoskeleton organization
J	0005737	cellular_component	cytoplasm
J	0005856	cellular_component	cytoskeleton
J	0005869	cellular_component	dynactin complex
J	0098958	biological_process	retrograde axonal transport of mitochondrion
J	1904115	cellular_component	axon cytoplasm
K	0003779	molecular_function	actin binding
K	0005737	cellular_component	cytoplasm
K	0005856	cellular_component	cytoskeleton
K	0008290	cellular_component	F-actin capping protein complex
K	0030036	biological_process	actin cytoskeleton organization
K	0051015	molecular_function	actin filament binding
K	0051016	biological_process	barbed-end actin filament capping
K	0051693	biological_process	actin filament capping
L	0000902	biological_process	cell morphogenesis
L	0003779	molecular_function	actin binding
L	0005737	cellular_component	cytoplasm
L	0005856	cellular_component	cytoskeleton
L	0007010	biological_process	cytoskeleton organization
L	0008290	cellular_component	F-actin capping protein complex
L	0010591	biological_process	regulation of lamellipodium assembly
L	0022604	biological_process	regulation of cell morphogenesis
L	0030017	cellular_component	sarcomere
L	0030036	biological_process	actin cytoskeleton organization
L	0051015	molecular_function	actin filament binding
L	0051016	biological_process	barbed-end actin filament capping
L	0051490	biological_process	negative regulation of filopodium assembly
L	0051693	biological_process	actin filament capping
L	0071203	cellular_component	WASH complex
U	0000775	cellular_component	chromosome, centromeric region
U	0000776	cellular_component	kinetochore
U	0005694	cellular_component	chromosome
U	0005737	cellular_component	cytoplasm
U	0005856	cellular_component	cytoskeleton
U	0005869	cellular_component	dynactin complex
U	0007052	biological_process	mitotic spindle organization
U	0070840	molecular_function	dynein complex binding
V	0000775	cellular_component	chromosome, centromeric region
V	0000776	cellular_component	kinetochore
V	0005694	cellular_component	chromosome
V	0005737	cellular_component	cytoplasm
V	0005856	cellular_component	cytoskeleton
V	0005869	cellular_component	dynactin complex
a	0005869	cellular_component	dynactin complex
a	0007017	biological_process	microtubule-based process
b	0005869	cellular_component	dynactin complex
b	0007017	biological_process	microtubule-based process
c	0005869	cellular_component	dynactin complex
c	0007017	biological_process	microtubule-based process
e	0007018	biological_process	microtubule-based movement
e	0008569	molecular_function	minus-end-directed microtubule motor activity
e	0030286	cellular_component	dynein complex
e	0045505	molecular_function	dynein intermediate chain binding
e	0051959	molecular_function	dynein light intermediate chain binding
f	0007018	biological_process	microtubule-based movement
f	0008569	molecular_function	minus-end-directed microtubule motor activity
f	0030286	cellular_component	dynein complex
f	0045505	molecular_function	dynein intermediate chain binding
f	0051959	molecular_function	dynein light intermediate chain binding
g	0003777	molecular_function	microtubule motor activity
g	0005515	molecular_function	protein binding
g	0005737	cellular_component	cytoplasm
g	0005813	cellular_component	centrosome
g	0005829	cellular_component	cytosol
g	0005856	cellular_component	cytoskeleton
g	0005868	cellular_component	cytoplasmic dynein complex
g	0005874	cellular_component	microtubule
g	0007018	biological_process	microtubule-based movement
g	0010970	biological_process	transport along microtubule
g	0030286	cellular_component	dynein complex
g	0031982	cellular_component	vesicle
g	0045503	molecular_function	dynein light chain binding
g	0045504	molecular_function	dynein heavy chain binding
h	0003777	molecular_function	microtubule motor activity
h	0005515	molecular_function	protein binding
h	0005737	cellular_component	cytoplasm
h	0005813	cellular_component	centrosome
h	0005829	cellular_component	cytosol
h	0005856	cellular_component	cytoskeleton
h	0005868	cellular_component	cytoplasmic dynein complex
h	0005874	cellular_component	microtubule
h	0007018	biological_process	microtubule-based movement
h	0010970	biological_process	transport along microtubule
h	0030286	cellular_component	dynein complex
h	0031982	cellular_component	vesicle
h	0045503	molecular_function	dynein light chain binding
h	0045504	molecular_function	dynein heavy chain binding
i	0000166	molecular_function	nucleotide binding
i	0000226	biological_process	microtubule cytoskeleton organization
i	0000776	cellular_component	kinetochore
i	0005524	molecular_function	ATP binding
i	0005737	cellular_component	cytoplasm
i	0005770	cellular_component	late endosome
i	0005813	cellular_component	centrosome
i	0005829	cellular_component	cytosol
i	0005856	cellular_component	cytoskeleton
i	0005868	cellular_component	cytoplasmic dynein complex
i	0005874	cellular_component	microtubule
i	0007018	biological_process	microtubule-based movement
i	0016020	cellular_component	membrane
i	0030286	cellular_component	dynein complex
i	0042802	molecular_function	identical protein binding
i	0045504	molecular_function	dynein heavy chain binding
i	0051642	biological_process	centrosome localization
i	1990090	biological_process	cellular response to nerve growth factor stimulus
j	0000166	molecular_function	nucleotide binding
j	0000226	biological_process	microtubule cytoskeleton organization
j	0000776	cellular_component	kinetochore
j	0005524	molecular_function	ATP binding
j	0005737	cellular_component	cytoplasm
j	0005770	cellular_component	late endosome
j	0005813	cellular_component	centrosome
j	0005829	cellular_component	cytosol
j	0005856	cellular_component	cytoskeleton
j	0005868	cellular_component	cytoplasmic dynein complex
j	0005874	cellular_component	microtubule
j	0007018	biological_process	microtubule-based movement
j	0016020	cellular_component	membrane
j	0030286	cellular_component	dynein complex
j	0042802	molecular_function	identical protein binding
j	0045504	molecular_function	dynein heavy chain binding
j	0051642	biological_process	centrosome localization
j	1990090	biological_process	cellular response to nerve growth factor stimulus
k	0003777	molecular_function	microtubule motor activity
k	0005515	molecular_function	protein binding
k	0005737	cellular_component	cytoplasm
k	0005813	cellular_component	centrosome
k	0005856	cellular_component	cytoskeleton
k	0005868	cellular_component	cytoplasmic dynein complex
k	0005874	cellular_component	microtubule
k	0005929	cellular_component	cilium
k	0007018	biological_process	microtubule-based movement
k	0007632	biological_process	visual behavior
k	0016020	cellular_component	membrane
k	0030286	cellular_component	dynein complex
k	0045505	molecular_function	dynein intermediate chain binding
k	0097542	cellular_component	ciliary tip
l	0003777	molecular_function	microtubule motor activity
l	0005515	molecular_function	protein binding
l	0005737	cellular_component	cytoplasm
l	0005813	cellular_component	centrosome
l	0005856	cellular_component	cytoskeleton
l	0005868	cellular_component	cytoplasmic dynein complex
l	0005874	cellular_component	microtubule
l	0005929	cellular_component	cilium
l	0007018	biological_process	microtubule-based movement
l	0007632	biological_process	visual behavior
l	0016020	cellular_component	membrane
l	0030286	cellular_component	dynein complex
l	0045505	molecular_function	dynein intermediate chain binding
l	0097542	cellular_component	ciliary tip
m	0007018	biological_process	microtubule-based movement
m	0008569	molecular_function	minus-end-directed microtubule motor activity
m	0030286	cellular_component	dynein complex
m	0045505	molecular_function	dynein intermediate chain binding
m	0051959	molecular_function	dynein light intermediate chain binding
n	0007018	biological_process	microtubule-based movement
n	0008569	molecular_function	minus-end-directed microtubule motor activity
n	0030286	cellular_component	dynein complex
n	0045505	molecular_function	dynein intermediate chain binding
n	0051959	molecular_function	dynein light intermediate chain binding
o	0003777	molecular_function	microtubule motor activity
o	0005515	molecular_function	protein binding
o	0005737	cellular_component	cytoplasm
o	0005813	cellular_component	centrosome
o	0005829	cellular_component	cytosol
o	0005856	cellular_component	cytoskeleton
o	0005868	cellular_component	cytoplasmic dynein complex
o	0005874	cellular_component	microtubule
o	0007018	biological_process	microtubule-based movement
o	0010970	biological_process	transport along microtubule
o	0030286	cellular_component	dynein complex
o	0031982	cellular_component	vesicle
o	0045503	molecular_function	dynein light chain binding
o	0045504	molecular_function	dynein heavy chain binding
p	0003777	molecular_function	microtubule motor activity
p	0005515	molecular_function	protein binding
p	0005737	cellular_component	cytoplasm
p	0005813	cellular_component	centrosome
p	0005829	cellular_component	cytosol
p	0005856	cellular_component	cytoskeleton
p	0005868	cellular_component	cytoplasmic dynein complex
p	0005874	cellular_component	microtubule
p	0007018	biological_process	microtubule-based movement
p	0010970	biological_process	transport along microtubule
p	0030286	cellular_component	dynein complex
p	0031982	cellular_component	vesicle
p	0045503	molecular_function	dynein light chain binding
p	0045504	molecular_function	dynein heavy chain binding
q	0000166	molecular_function	nucleotide binding
q	0000226	biological_process	microtubule cytoskeleton organization
q	0000776	cellular_component	kinetochore
q	0005524	molecular_function	ATP binding
q	0005737	cellular_component	cytoplasm
q	0005770	cellular_component	late endosome
q	0005813	cellular_component	centrosome
q	0005829	cellular_component	cytosol
q	0005856	cellular_component	cytoskeleton
q	0005868	cellular_component	cytoplasmic dynein complex
q	0005874	cellular_component	microtubule
q	0007018	biological_process	microtubule-based movement
q	0016020	cellular_component	membrane
q	0030286	cellular_component	dynein complex
q	0042802	molecular_function	identical protein binding
q	0045504	molecular_function	dynein heavy chain binding
q	0051642	biological_process	centrosome localization
q	1990090	biological_process	cellular response to nerve growth factor stimulus
r	0000166	molecular_function	nucleotide binding
r	0000226	biological_process	microtubule cytoskeleton organization
r	0000776	cellular_component	kinetochore
r	0005524	molecular_function	ATP binding
r	0005737	cellular_component	cytoplasm
r	0005770	cellular_component	late endosome
r	0005813	cellular_component	centrosome
r	0005829	cellular_component	cytosol
r	0005856	cellular_component	cytoskeleton
r	0005868	cellular_component	cytoplasmic dynein complex
r	0005874	cellular_component	microtubule
r	0007018	biological_process	microtubule-based movement
r	0016020	cellular_component	membrane
r	0030286	cellular_component	dynein complex
r	0042802	molecular_function	identical protein binding
r	0045504	molecular_function	dynein heavy chain binding
r	0051642	biological_process	centrosome localization
r	1990090	biological_process	cellular response to nerve growth factor stimulus
s	0003777	molecular_function	microtubule motor activity
s	0005515	molecular_function	protein binding
s	0005737	cellular_component	cytoplasm
s	0005813	cellular_component	centrosome
s	0005856	cellular_component	cytoskeleton
s	0005868	cellular_component	cytoplasmic dynein complex
s	0005874	cellular_component	microtubule
s	0005929	cellular_component	cilium
s	0007018	biological_process	microtubule-based movement
s	0007632	biological_process	visual behavior
s	0016020	cellular_component	membrane
s	0030286	cellular_component	dynein complex
s	0045505	molecular_function	dynein intermediate chain binding
s	0097542	cellular_component	ciliary tip
t	0003777	molecular_function	microtubule motor activity
t	0005515	molecular_function	protein binding
t	0005737	cellular_component	cytoplasm
t	0005813	cellular_component	centrosome
t	0005856	cellular_component	cytoskeleton
t	0005868	cellular_component	cytoplasmic dynein complex
t	0005874	cellular_component	microtubule
t	0005929	cellular_component	cilium
t	0007018	biological_process	microtubule-based movement
t	0007632	biological_process	visual behavior
t	0016020	cellular_component	membrane
t	0030286	cellular_component	dynein complex
t	0045505	molecular_function	dynein intermediate chain binding
t	0097542	cellular_component	ciliary tip

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	binding site for residue ADP A 800

Chain	Residue
A	GLY17
A	GLU219
A	GLY302
A	GLY303
A	SER304
A	PHE307
A	SER18
A	VAL20
A	LYS22
A	GLN142
A	SER160
A	GLY161
A	ASP162
A	LYS218

site_id	AC2
Number of Residues	12
Details	binding site for residue ADP B 800

Chain	Residue
B	ASN16
B	GLY17
B	SER18
B	VAL20
B	LYS22
B	GLY161
B	ASP162
B	LYS218
B	GLU219
B	GLY303
B	SER304
B	PHE307

site_id	AC3
Number of Residues	11
Details	binding site for residue ADP C 800

Chain	Residue
C	GLY17
C	SER18
C	GLY19
C	VAL20
C	LYS22
C	GLY161
C	ASP162
C	LYS218
C	GLU219
C	GLY303
C	PHE307

site_id	AC4
Number of Residues	16
Details	binding site for residue ADP D 800

Chain	Residue
D	GLY17
D	SER18
D	GLY19
D	VAL20
D	LYS22
D	SER160
D	GLY161
D	ASP162
D	LYS218
D	GLU219
D	GLY302
D	GLY303
D	SER304
D	LEU306
D	PHE307
D	LEU337

site_id	AC5
Number of Residues	14
Details	binding site for residue ADP E 800

Chain	Residue
E	GLY17
E	SER18
E	GLY19
E	VAL20
E	LYS22
E	GLY161
E	ASP162
E	LYS218
E	GLU219
E	GLY302
E	GLY303
E	SER304
E	LEU306
E	PHE307

site_id	AC6
Number of Residues	14
Details	binding site for residue ADP F 800

Chain	Residue
F	GLY17
F	VAL20
F	LYS22
F	SER160
F	GLY161
F	ASP162
F	LYS215
F	LYS218
F	GLU219
F	GLY302
F	GLY303
F	SER304
F	LEU306
F	LEU337

site_id	AC7
Number of Residues	16
Details	binding site for residue ADP G 800

Chain	Residue
G	GLY17
G	SER18
G	GLY19
G	VAL20
G	LYS22
G	GLY161
G	ASP162
G	LYS215
G	LYS218
G	GLU219
G	GLY302
G	GLY303
G	SER304
G	LEU306
G	PHE307
G	LEU337

site_id	AC8
Number of Residues	16
Details	binding site for residue ATP H 401

Chain	Residue
H	SER14
H	GLY15
H	MET16
H	LYS18
H	GLN137
H	GLY156
H	ASP157
H	ARG210
H	LYS213
H	GLU214
H	GLY302
H	THR303
H	MET305
H	TYR306
H	ASP11
H	GLY13

site_id	AC9
Number of Residues	14
Details	binding site for residue ADP I 800

Chain	Residue
I	GLY17
I	SER18
I	GLY19
I	VAL20
I	GLY161
I	ASP162
I	LYS215
I	LYS218
I	GLU219
I	GLY302
I	GLY303
I	SER304
I	LEU306
I	PHE307

site_id	AD1
Number of Residues	12
Details	binding site for residue ADP J 800

Chain	Residue
J	GLY21
J	GLU22
J	ALA23
J	PHE24
J	LYS26
J	GLY142
J	TYR143
J	LYS211
J	GLY308
J	THR309
J	MET311
J	ASN351

site_id	AD2
Number of Residues	9
Details	binding site for Di-peptide ASP n 558 and GLU n 561

Chain	Residue
n	ASP554
n	GLU555
n	ARG556
n	ILE557
n	ARG559
n	VAL560
n	THR562
n	ARG563
n	ILE564

Functional Information from PROSITE/UniProt

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
H	TYR53-GLY63

site_id	PS00231
Number of Residues	6
Details	F_ACTIN_CAPPING_BETA F-actin capping protein beta subunit signature. CDYNRD

Chain	Residue	Details
L	CYS62-ASP67

site_id	PS00748
Number of Residues	9
Details	F_ACTIN_CAPPING_A_1 F-actin capping protein alpha subunit signature 1. VHYYEDGNV

Chain	Residue	Details
K	VAL196-VAL204

site_id	PS00749
Number of Residues	11
Details	F_ACTIN_CAPPING_A_2 F-actin capping protein alpha subunit signature 2. KaLRRqLPVTR

Chain	Residue	Details
K	LYS256-ARG266

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPrkNR

Chain	Residue	Details
A	LEU109-ARG121
H	LEU104-ARG116

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WVSKkEYEE

Chain	Residue	Details
A	TRP357-GLU365
H	TRP356-GLU364

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	MOD_RES: N-acetylalanine => ECO:0000250\|UniProtKB:P62628

Chain	Residue	Details
k	ALA2
l	ALA2
s	ALA2
t	ALA2
E	MET1
F	MET1
G	MET1
I	MET1

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17081983, ECO:0007744\|PubMed:18669648, ECO:0007744\|PubMed:18691976, ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:21406692, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
i	SER194
j	SER194
q	SER194
r	SER194

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18669648

Chain	Residue	Details
i	SER383
j	SER383
q	SER383
r	SER383

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:20068231

Chain	Residue	Details
i	SER391
j	SER391
q	SER391
r	SER391

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Omega-N-methylarginine => ECO:0007744\|PubMed:24129315

Chain	Residue	Details
i	ARG397
j	ARG397
q	ARG397
r	ARG397

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:20068231

Chain	Residue	Details
i	THR441
j	THR441
q	THR441
r	THR441

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18669648, ECO:0007744\|PubMed:20068231

Chain	Residue	Details
i	SER443
j	SER443
q	SER443
r	SER443

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:16964243, ECO:0007744\|PubMed:18669648, ECO:0007744\|PubMed:18691976, ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
i	SER446
j	SER446
q	SER446
r	SER446

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)