6EZC
Crystal Structure of human tRNA-dihydrouridine(20) synthase catalytic domain E294K Q305K double mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | tRNA-dihydrouridine(20) synthase [NAD(P)+]-like | polymer | 327 | 36806.5 | 1 | UniProt (Q9NX74) Pfam (PF01207) | Homo sapiens (Human) | Dihydrouridine synthase 2,Up-regulated in lung cancer protein 8,URLC8,tRNA-dihydrouridine synthase 2-like,hDUS2 |
| 2 | B (A) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) | |||
| 3 | C, D, E, J, K (A) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 4 | F (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 5 | G, H (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 6 | I (A) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 7 | L (A) | water | water | 18.0 | 230 | Chemie (HOH) |
Sequence modifications
A: 14 - 333 (UniProt: Q9NX74)
| PDB | External Database | Details |
|---|---|---|
| Met 7 | - | initiating methionine |
| His 8 | - | expression tag |
| His 9 | - | expression tag |
| His 10 | - | expression tag |
| His 11 | - | expression tag |
| His 12 | - | expression tag |
| His 13 | - | expression tag |
| Lys 294 | Glu 294 | engineered mutation |
| Lys 305 | Gln 305 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 36806.5 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1306.5 | |
| All* | Total formula weight | 38112.9 |
