6EZC

Crystal Structure of human tRNA-dihydrouridine(20) synthase catalytic domain E294K Q305K double mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980066
Spacegroup name	P 21 21 21
Unit cell lengths	45.990, 84.660, 100.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.330 - 2.000
R-factor	0.184
Rwork	0.182
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xp7
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS (20151015)
Data scaling software	STARANISO (1.10.15)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.170	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.161	2.521
Rmeas	0.170	2.665
Number of reflections	22677	1135
<I/σ(I)>	10.1	1.1
Completeness [%]	89.9	43.5
Redundancy	9.3	8.9
CC(1/2)	0.997	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	292	30% PEG 2000 MME 200 mM ammonium sulfate 50 mM sodium acetate