6EZA
Crystal Structure of human tRNA-dihydrouridine(20) synthase catalytic domain E294K mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | tRNA-dihydrouridine(20) synthase [NAD(P)+]-like | polymer | 327 | 36805.4 | 2 | UniProt (Q9NX74) Pfam (PF01207) | Homo sapiens (Human) | Dihydrouridine synthase 2,Up-regulated in lung cancer protein 8,URLC8,tRNA-dihydrouridine synthase 2-like,hDUS2 |
| 2 | C, L (A, B) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 2 | Chemie (FMN) | |||
| 3 | D, E, F, H, K... (A, B) | SULFATE ION | non-polymer | 96.1 | 8 | Chemie (SO4) | |||
| 4 | G, I (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 5 | J, P (B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 6 | Q, R (A, B) | water | water | 18.0 | 359 | Chemie (HOH) |
Sequence modifications
A, B: 14 - 333 (UniProt: Q9NX74)
| PDB | External Database | Details |
|---|---|---|
| Met 7 | - | initiating methionine |
| His 8 | - | expression tag |
| His 9 | - | expression tag |
| His 10 | - | expression tag |
| His 11 | - | expression tag |
| His 12 | - | expression tag |
| His 13 | - | expression tag |
| Lys 294 | Glu 294 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 73610.9 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1936.3 | |
| All* | Total formula weight | 75547.1 |
