6EZA

Crystal Structure of human tRNA-dihydrouridine(20) synthase catalytic domain E294K mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like	polymer	327	36805.4	2	UniProt (Q9NX74) Pfam (PF01207) In PDB	Homo sapiens (Human)	Dihydrouridine synthase 2,Up-regulated in lung cancer protein 8,URLC8,tRNA-dihydrouridine synthase 2-like,hDUS2
2	A, B	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3	A, B	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
4	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6		water	water		18.0	359	Chemie (HOH)

Sequence modifications

A, B: 14 - 333 (UniProt: Q9NX74)

PDB	External Database	Details
Met 7	-	initiating methionine
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
Lys 294	Glu 294	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73610.9
Non-Polymers*	Number of molecules	14
	Total formula weight	1936.3
All*	Total formula weight	75547.1

*Water molecules are not included.