6EVK

Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter and cap analogue m7GTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polymerase acidic protein	polymer	738	85490.9	1	UniProt (H6QM92) Pfam (PF00603)	Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
2	B (B)	RNA-directed RNA polymerase catalytic subunit	polymer	776	87936.3	1	UniProt (H6QM91) Pfam (PF00602)	Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
3	C (C)	Polymerase basic protein 2	polymer	809	91027.1	1	UniProt (H6QM90) Pfam (PF20947) Pfam (PF20948) Pfam (PF20949) Pfam (PF20950) Pfam (PF00604) Pfam (PF20951) Pfam (PF20952)	Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
4	D (R)	RNA (5'-R(*UP*AP*UP*AP*CP*CP*UP*CP*UP*GP*CP*UP*U)-3')	polymer	18	5584.3	1		Influenza A virus
5	E (V)	RNA (5'-R(P*AP*GP*UP*AP*GP*UP*AP*AP*CP*AP*AP*GP*AP*GP*GP*G)-3')	polymer	16	5248.2	1		Influenza A virus
6	F (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
7	G, H, I, J, K... (A, B, C)	PHOSPHATE ION	non-polymer		95.0	12	Chemie (PO4)
8	Q (C)	7-METHYL-GUANOSINE-5'-TRIPHOSPHATE	non-polymer		538.2	1	Chemie (MGP)
9	T, U, V, W, X (A, B, C, R, V)	water	water		18.0	38	Chemie (HOH)

Sequence modifications

A: 1 - 713 (UniProt: H6QM92)

PDB	External Database	Details
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 714	-	expression tag
Ser 715	-	expression tag
Gly 716	-	expression tag
Ser 717	-	expression tag
Gly 718	-	expression tag
Glu 719	-	expression tag
Asn 720	-	expression tag
Leu 721	-	expression tag
Tyr 722	-	expression tag
Phe 723	-	expression tag
Gln 724	-	expression tag

B: 1 - 756 (UniProt: H6QM91)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 757	-	expression tag
Ser 758	-	expression tag
Gly 759	-	expression tag
Ser 760	-	expression tag
Gly 761	-	expression tag
Glu 762	-	expression tag
Asn 763	-	expression tag
Leu 764	-	expression tag
Tyr 765	-	expression tag
Phe 766	-	expression tag
Gln 767	-	expression tag

C: 1 - 760 (UniProt: H6QM90)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 761	-	expression tag
Trp 762	-	expression tag
Ser 763	-	expression tag
His 764	-	expression tag
Pro 765	-	expression tag
Gln 766	-	expression tag
Phe 767	-	expression tag
Glu 768	-	expression tag
Lys 769	-	expression tag
Gly 770	-	expression tag
Gly 771	-	expression tag
Gly 772	-	expression tag
Ser 773	-	expression tag
Gly 774	-	expression tag
Gly 775	-	expression tag
Gly 776	-	expression tag
Ser 777	-	expression tag
Gly 778	-	expression tag
Gly 779	-	expression tag
Ser 780	-	expression tag
Ala 781	-	expression tag
Trp 782	-	expression tag
Ser 783	-	expression tag
His 784	-	expression tag
Pro 785	-	expression tag
Gln 786	-	expression tag
Phe 787	-	expression tag
Glu 788	-	expression tag
Lys 789	-	expression tag
Gly 790	-	expression tag
Arg 791	-	expression tag
Ser 792	-	expression tag
Gly 793	-	expression tag
Gly 794	-	expression tag
Glu 795	-	expression tag
Asn 796	-	expression tag
Leu 797	-	expression tag
Tyr 798	-	expression tag
Phe 799	-	expression tag
Gln 800	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	275286.9
Non-Polymers*	Number of molecules	14
	Total formula weight	1743.3
All*	Total formula weight	277030.2

*Water molecules are not included.