6EVK
Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter and cap analogue m7GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-05 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 269.340, 148.700, 88.510 |
Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.900 |
R-factor | 0.23592 |
Rwork | 0.234 |
R-free | 0.27294 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wsb |
RMSD bond length | 0.008 |
RMSD bond angle | 1.139 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmeas | 0.115 | 1.380 |
Number of reflections | 76144 | |
<I/σ(I)> | 10.75 | 1.08 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 4.63 | 4.65 |
CC(1/2) | 0.998 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 281 | Bat influenza polymerase protein in 50 mM HEPES-NaOH, 500 mM NaCl, 5 % glycerol, 2 mM TCEP, pH = 7.5 was adjusted to a concentration of 10 mg per ml, mixed in a 1:1 ratio with vRNA, which was an equimolar mixture of nucleotides 1-16 from the 5 prime end and nucleotides 1-18 or 3-18 from the 3 prime end. Protein-RNA with the addition of 5 mM m7GTP was mixed with mother liquor containing 0.7-1.5 M sodium-potassium phosphate at pH 5.0 |