6EG8

Structure of the GDP-bound Gs heterotrimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, F	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37728.2	4	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
2	C, E, G, H	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	4	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
3	I, J, K, L	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	381	44333.1	4	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
4	I, J, K, L	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP)
5	I, J, K, L	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6		water	water		18.0	87	Chemie (HOH)

Sequence modifications

A, B, D, F: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

C, E, G, H: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

I, J, K, L: 4 - 394 (UniProt: P63092)

PDB	External Database	Details
Gly 0	-	expression tag
Pro 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	359625.2
Non-Polymers*	Number of molecules	8
	Total formula weight	1870.0
All*	Total formula weight	361495.2

*Water molecules are not included.