Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6EG8

Structure of the GDP-bound Gs heterotrimer

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001750	cellular_component	photoreceptor outer segment
A	0003924	molecular_function	GTPase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005765	cellular_component	lysosomal membrane
A	0005829	cellular_component	cytosol
A	0005834	cellular_component	heterotrimeric G-protein complex
A	0005886	cellular_component	plasma membrane
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
A	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
A	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
A	0007265	biological_process	Ras protein signal transduction
A	0008283	biological_process	cell population proliferation
A	0016020	cellular_component	membrane
A	0030159	molecular_function	signaling receptor complex adaptor activity
A	0044877	molecular_function	protein-containing complex binding
A	0045202	cellular_component	synapse
A	0050909	biological_process	sensory perception of taste
A	0051020	molecular_function	GTPase binding
A	0060041	biological_process	retina development in camera-type eye
A	0070062	cellular_component	extracellular exosome
A	0071380	biological_process	cellular response to prostaglandin E stimulus
A	0071870	biological_process	cellular response to catecholamine stimulus
A	0097381	cellular_component	photoreceptor disc membrane
A	1903561	cellular_component	extracellular vesicle
B	0001750	cellular_component	photoreceptor outer segment
B	0003924	molecular_function	GTPase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005765	cellular_component	lysosomal membrane
B	0005829	cellular_component	cytosol
B	0005834	cellular_component	heterotrimeric G-protein complex
B	0005886	cellular_component	plasma membrane
B	0007165	biological_process	signal transduction
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
B	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
B	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
B	0007265	biological_process	Ras protein signal transduction
B	0008283	biological_process	cell population proliferation
B	0016020	cellular_component	membrane
B	0030159	molecular_function	signaling receptor complex adaptor activity
B	0044877	molecular_function	protein-containing complex binding
B	0045202	cellular_component	synapse
B	0050909	biological_process	sensory perception of taste
B	0051020	molecular_function	GTPase binding
B	0060041	biological_process	retina development in camera-type eye
B	0070062	cellular_component	extracellular exosome
B	0071380	biological_process	cellular response to prostaglandin E stimulus
B	0071870	biological_process	cellular response to catecholamine stimulus
B	0097381	cellular_component	photoreceptor disc membrane
B	1903561	cellular_component	extracellular vesicle
C	0005515	molecular_function	protein binding
C	0005834	cellular_component	heterotrimeric G-protein complex
C	0005886	cellular_component	plasma membrane
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
C	0016020	cellular_component	membrane
C	0031681	molecular_function	G-protein beta-subunit binding
C	0048144	biological_process	fibroblast proliferation
C	0070062	cellular_component	extracellular exosome
C	0071380	biological_process	cellular response to prostaglandin E stimulus
C	0071870	biological_process	cellular response to catecholamine stimulus
D	0001750	cellular_component	photoreceptor outer segment
D	0003924	molecular_function	GTPase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005765	cellular_component	lysosomal membrane
D	0005829	cellular_component	cytosol
D	0005834	cellular_component	heterotrimeric G-protein complex
D	0005886	cellular_component	plasma membrane
D	0007165	biological_process	signal transduction
D	0007186	biological_process	G protein-coupled receptor signaling pathway
D	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
D	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
D	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
D	0007265	biological_process	Ras protein signal transduction
D	0008283	biological_process	cell population proliferation
D	0016020	cellular_component	membrane
D	0030159	molecular_function	signaling receptor complex adaptor activity
D	0044877	molecular_function	protein-containing complex binding
D	0045202	cellular_component	synapse
D	0050909	biological_process	sensory perception of taste
D	0051020	molecular_function	GTPase binding
D	0060041	biological_process	retina development in camera-type eye
D	0070062	cellular_component	extracellular exosome
D	0071380	biological_process	cellular response to prostaglandin E stimulus
D	0071870	biological_process	cellular response to catecholamine stimulus
D	0097381	cellular_component	photoreceptor disc membrane
D	1903561	cellular_component	extracellular vesicle
E	0005515	molecular_function	protein binding
E	0005834	cellular_component	heterotrimeric G-protein complex
E	0005886	cellular_component	plasma membrane
E	0007165	biological_process	signal transduction
E	0007186	biological_process	G protein-coupled receptor signaling pathway
E	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
E	0016020	cellular_component	membrane
E	0031681	molecular_function	G-protein beta-subunit binding
E	0048144	biological_process	fibroblast proliferation
E	0070062	cellular_component	extracellular exosome
E	0071380	biological_process	cellular response to prostaglandin E stimulus
E	0071870	biological_process	cellular response to catecholamine stimulus
F	0001750	cellular_component	photoreceptor outer segment
F	0003924	molecular_function	GTPase activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005765	cellular_component	lysosomal membrane
F	0005829	cellular_component	cytosol
F	0005834	cellular_component	heterotrimeric G-protein complex
F	0005886	cellular_component	plasma membrane
F	0007165	biological_process	signal transduction
F	0007186	biological_process	G protein-coupled receptor signaling pathway
F	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
F	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
F	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
F	0007265	biological_process	Ras protein signal transduction
F	0008283	biological_process	cell population proliferation
F	0016020	cellular_component	membrane
F	0030159	molecular_function	signaling receptor complex adaptor activity
F	0044877	molecular_function	protein-containing complex binding
F	0045202	cellular_component	synapse
F	0050909	biological_process	sensory perception of taste
F	0051020	molecular_function	GTPase binding
F	0060041	biological_process	retina development in camera-type eye
F	0070062	cellular_component	extracellular exosome
F	0071380	biological_process	cellular response to prostaglandin E stimulus
F	0071870	biological_process	cellular response to catecholamine stimulus
F	0097381	cellular_component	photoreceptor disc membrane
F	1903561	cellular_component	extracellular vesicle
G	0005515	molecular_function	protein binding
G	0005834	cellular_component	heterotrimeric G-protein complex
G	0005886	cellular_component	plasma membrane
G	0007165	biological_process	signal transduction
G	0007186	biological_process	G protein-coupled receptor signaling pathway
G	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
G	0016020	cellular_component	membrane
G	0031681	molecular_function	G-protein beta-subunit binding
G	0048144	biological_process	fibroblast proliferation
G	0070062	cellular_component	extracellular exosome
G	0071380	biological_process	cellular response to prostaglandin E stimulus
G	0071870	biological_process	cellular response to catecholamine stimulus
H	0005515	molecular_function	protein binding
H	0005834	cellular_component	heterotrimeric G-protein complex
H	0005886	cellular_component	plasma membrane
H	0007165	biological_process	signal transduction
H	0007186	biological_process	G protein-coupled receptor signaling pathway
H	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
H	0016020	cellular_component	membrane
H	0031681	molecular_function	G-protein beta-subunit binding
H	0048144	biological_process	fibroblast proliferation
H	0070062	cellular_component	extracellular exosome
H	0071380	biological_process	cellular response to prostaglandin E stimulus
H	0071870	biological_process	cellular response to catecholamine stimulus
I	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
I	0003091	biological_process	renal water homeostasis
I	0003924	molecular_function	GTPase activity
I	0003925	molecular_function	G protein activity
I	0005515	molecular_function	protein binding
I	0005525	molecular_function	GTP binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005834	cellular_component	heterotrimeric G-protein complex
I	0005886	cellular_component	plasma membrane
I	0007165	biological_process	signal transduction
I	0007186	biological_process	G protein-coupled receptor signaling pathway
I	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
I	0007190	biological_process	activation of adenylate cyclase activity
I	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
I	0007608	biological_process	sensory perception of smell
I	0010856	molecular_function	adenylate cyclase activator activity
I	0016020	cellular_component	membrane
I	0019001	molecular_function	guanyl nucleotide binding
I	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
I	0031748	molecular_function	D1 dopamine receptor binding
I	0032588	cellular_component	trans-Golgi network membrane
I	0046872	molecular_function	metal ion binding
I	0046907	biological_process	intracellular transport
I	0048589	biological_process	developmental growth
I	0050796	biological_process	regulation of insulin secretion
I	0050890	biological_process	cognition
I	0060348	biological_process	bone development
I	0060789	biological_process	hair follicle placode formation
I	0070062	cellular_component	extracellular exosome
I	0070527	biological_process	platelet aggregation
I	0071377	biological_process	cellular response to glucagon stimulus
I	0071380	biological_process	cellular response to prostaglandin E stimulus
I	0071870	biological_process	cellular response to catecholamine stimulus
I	0071880	biological_process	adenylate cyclase-activating adrenergic receptor signaling pathway
I	0120162	biological_process	positive regulation of cold-induced thermogenesis
J	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
J	0003091	biological_process	renal water homeostasis
J	0003924	molecular_function	GTPase activity
J	0003925	molecular_function	G protein activity
J	0005515	molecular_function	protein binding
J	0005525	molecular_function	GTP binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005834	cellular_component	heterotrimeric G-protein complex
J	0005886	cellular_component	plasma membrane
J	0007165	biological_process	signal transduction
J	0007186	biological_process	G protein-coupled receptor signaling pathway
J	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
J	0007190	biological_process	activation of adenylate cyclase activity
J	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
J	0007608	biological_process	sensory perception of smell
J	0010856	molecular_function	adenylate cyclase activator activity
J	0016020	cellular_component	membrane
J	0019001	molecular_function	guanyl nucleotide binding
J	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
J	0031748	molecular_function	D1 dopamine receptor binding
J	0032588	cellular_component	trans-Golgi network membrane
J	0046872	molecular_function	metal ion binding
J	0046907	biological_process	intracellular transport
J	0048589	biological_process	developmental growth
J	0050796	biological_process	regulation of insulin secretion
J	0050890	biological_process	cognition
J	0060348	biological_process	bone development
J	0060789	biological_process	hair follicle placode formation
J	0070062	cellular_component	extracellular exosome
J	0070527	biological_process	platelet aggregation
J	0071377	biological_process	cellular response to glucagon stimulus
J	0071380	biological_process	cellular response to prostaglandin E stimulus
J	0071870	biological_process	cellular response to catecholamine stimulus
J	0071880	biological_process	adenylate cyclase-activating adrenergic receptor signaling pathway
J	0120162	biological_process	positive regulation of cold-induced thermogenesis
K	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
K	0003091	biological_process	renal water homeostasis
K	0003924	molecular_function	GTPase activity
K	0003925	molecular_function	G protein activity
K	0005515	molecular_function	protein binding
K	0005525	molecular_function	GTP binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005834	cellular_component	heterotrimeric G-protein complex
K	0005886	cellular_component	plasma membrane
K	0007165	biological_process	signal transduction
K	0007186	biological_process	G protein-coupled receptor signaling pathway
K	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
K	0007190	biological_process	activation of adenylate cyclase activity
K	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
K	0007608	biological_process	sensory perception of smell
K	0010856	molecular_function	adenylate cyclase activator activity
K	0016020	cellular_component	membrane
K	0019001	molecular_function	guanyl nucleotide binding
K	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
K	0031748	molecular_function	D1 dopamine receptor binding
K	0032588	cellular_component	trans-Golgi network membrane
K	0046872	molecular_function	metal ion binding
K	0046907	biological_process	intracellular transport
K	0048589	biological_process	developmental growth
K	0050796	biological_process	regulation of insulin secretion
K	0050890	biological_process	cognition
K	0060348	biological_process	bone development
K	0060789	biological_process	hair follicle placode formation
K	0070062	cellular_component	extracellular exosome
K	0070527	biological_process	platelet aggregation
K	0071377	biological_process	cellular response to glucagon stimulus
K	0071380	biological_process	cellular response to prostaglandin E stimulus
K	0071870	biological_process	cellular response to catecholamine stimulus
K	0071880	biological_process	adenylate cyclase-activating adrenergic receptor signaling pathway
K	0120162	biological_process	positive regulation of cold-induced thermogenesis
L	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
L	0003091	biological_process	renal water homeostasis
L	0003924	molecular_function	GTPase activity
L	0003925	molecular_function	G protein activity
L	0005515	molecular_function	protein binding
L	0005525	molecular_function	GTP binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005834	cellular_component	heterotrimeric G-protein complex
L	0005886	cellular_component	plasma membrane
L	0007165	biological_process	signal transduction
L	0007186	biological_process	G protein-coupled receptor signaling pathway
L	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
L	0007190	biological_process	activation of adenylate cyclase activity
L	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
L	0007608	biological_process	sensory perception of smell
L	0010856	molecular_function	adenylate cyclase activator activity
L	0016020	cellular_component	membrane
L	0019001	molecular_function	guanyl nucleotide binding
L	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
L	0031748	molecular_function	D1 dopamine receptor binding
L	0032588	cellular_component	trans-Golgi network membrane
L	0046872	molecular_function	metal ion binding
L	0046907	biological_process	intracellular transport
L	0048589	biological_process	developmental growth
L	0050796	biological_process	regulation of insulin secretion
L	0050890	biological_process	cognition
L	0060348	biological_process	bone development
L	0060789	biological_process	hair follicle placode formation
L	0070062	cellular_component	extracellular exosome
L	0070527	biological_process	platelet aggregation
L	0071377	biological_process	cellular response to glucagon stimulus
L	0071380	biological_process	cellular response to prostaglandin E stimulus
L	0071870	biological_process	cellular response to catecholamine stimulus
L	0071880	biological_process	adenylate cyclase-activating adrenergic receptor signaling pathway
L	0120162	biological_process	positive regulation of cold-induced thermogenesis

Functional Information from PDB Data

site_id	AC1
Number of Residues	21
Details	binding site for residue GDP I 401

Chain	Residue
I	ALA48
I	ARG199
I	CYS200
I	ARG201
I	ASN292
I	LYS293
I	ASP295
I	LEU296
I	CYS365
I	ALA366
I	VAL367
I	GLU50
I	MG402
I	HOH504
I	SER51
I	GLY52
I	LYS53
I	SER54
I	THR55
I	ASP173
I	LEU198

site_id	AC2
Number of Residues	5
Details	binding site for residue MG I 402

Chain	Residue
I	SER54
I	THR204
I	GDP401
I	HOH503
I	HOH504

site_id	AC3
Number of Residues	21
Details	binding site for residue GDP J 401

Chain	Residue
J	GLY49
J	GLU50
J	SER51
J	GLY52
J	LYS53
J	SER54
J	THR55
J	ASP173
J	LEU198
J	ARG199
J	CYS200
J	ARG201
J	ASN292
J	LYS293
J	ASP295
J	LEU296
J	CYS365
J	ALA366
J	MG402
J	HOH507
J	HOH514

site_id	AC4
Number of Residues	6
Details	binding site for residue MG J 402

Chain	Residue
J	SER54
J	THR204
J	GDP401
J	HOH507
J	HOH513
J	HOH515

site_id	AC5
Number of Residues	21
Details	binding site for residue GDP K 401

Chain	Residue
K	GLY49
K	GLU50
K	SER51
K	GLY52
K	LYS53
K	SER54
K	THR55
K	ASP173
K	ARG199
K	CYS200
K	ARG201
K	ASN292
K	LYS293
K	ASP295
K	LEU296
K	CYS365
K	ALA366
K	VAL367
K	MG402
K	HOH503
K	HOH505

site_id	AC6
Number of Residues	7
Details	binding site for residue MG K 402

Chain	Residue
K	SER54
K	ARG201
K	VAL202
K	THR204
K	GDP401
K	HOH503
K	HOH505

site_id	AC7
Number of Residues	22
Details	binding site for residue GDP L 401

Chain	Residue
L	MG402
L	HOH504
L	HOH507
L	HOH508
L	GLU50
L	SER51
L	GLY52
L	LYS53
L	SER54
L	THR55
L	ASP173
L	LEU198
L	ARG199
L	CYS200
L	ARG201
L	ASN292
L	LYS293
L	ASP295
L	LEU296
L	CYS365
L	ALA366
L	VAL367

site_id	AC8
Number of Residues	5
Details	binding site for residue MG L 402

Chain	Residue
L	SER54
L	THR204
L	GDP401
L	HOH507
L	HOH508

Functional Information from PROSITE/UniProt

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS

Chain	Residue	Details
A	LEU70-SER84
A	ILE157-ILE171
A	LEU285-ALA299

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	28
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P04896

Chain	Residue	Details
K	LYS211
K	ASN218
K	CYS237
K	SER306
K	ARG380
L	GLY47
L	SER54
L	LYS211
L	ASN218
L	CYS237
L	SER306
L	ARG380
I	GLY47
I	SER54
I	LYS211
I	ASN218
I	CYS237
I	SER306
I	ARG380
J	GLY47
J	SER54
J	LYS211
J	ASN218
J	CYS237
J	SER306
J	ARG380
K	GLY47
K	SER54

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: ADP-ribosylarginine; by cholera toxin => ECO:0000250

Chain	Residue	Details
I	ASP215
J	ASP215
K	ASP215
L	ASP215

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:20068231

Chain	Residue	Details
I	ALA366
J	ALA366
K	ALA366
L	ALA366

site_id	SWS_FT_FI4
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Chain	Residue	Details
K	GLU314
L	GLU314
I	GLU314
J	GLU314

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)