6ECH

Pyruvate Kinase Isoform L-type with phosphorylated Ser12 (pS12) in complex with FBP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Pyruvate kinase PKLR	polymer	550	59910.5	4	UniProt (P12928) Pfam (PF00224) Pfam (PF02887) In PDB	Rattus norvegicus (Rat)	L-PK,Pyruvate kinase isozymes L/R
2	A, C, B, D	1,6-di-O-phosphono-beta-D-fructofuranose	non-polymer		340.1	4	Chemie (FBP)
3	D, A, C, B	MAGNESIUM ION	non-polymer		24.3	13	Chemie (MG)
4	D, A, B	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	3	Chemie (ATP)
5	B, D, A, C	OXALATE ION	non-polymer		88.0	4	Chemie (OXL)
6	B, D, A, C	1,2-ETHANEDIOL	non-polymer		62.1	33	Chemie (EDO)
7	D, A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
8	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
9	B	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
10	D	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
11		water	water		18.0	1201	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 543 (UniProt: P12928)

PDB	External Database	Details
His 0	-	expression tag
His 544	-	expression tag
His 545	-	expression tag
His 546	-	expression tag
His 547	-	expression tag
His 548	-	expression tag
His 549	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	239641.8
Non-Polymers*	Number of molecules	62
	Total formula weight	6190.4
All*	Total formula weight	245832.2

*Water molecules are not included.