6E8E

Crystal structure of the Escherichia coli sliding clamp-MutL complex.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta sliding clamp,DNA mismatch repair protein MutL	polymer	517	57098.8	1	UniProt (P0A988) UniProt (A7ZV39) Pfam (PF00712) Pfam (PF02767) Pfam (PF02768) Pfam (PF08676) UniProt (by SIFTS) (P23367)	Escherichia coli (strain K12)	Sliding clamp,Beta-clamp processivity factor,DNA polymerase III beta sliding clamp subunit
2	B (B)	Beta sliding clamp,DNA mismatch repair protein MutL	polymer	517	57082.8	1	UniProt (P0A988) UniProt (A7ZV39) Pfam (PF00712) Pfam (PF02767) Pfam (PF02768) Pfam (PF08676) UniProt (by SIFTS) (P23367)	Escherichia coli (strain K12)	Sliding clamp,Beta-clamp processivity factor,DNA polymerase III beta sliding clamp subunit
3	C, D, E, F, J... (A, B)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
4	G, H, I, L, M (A, B)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
5	N, O (A, B)	water	water		18.0	191	Chemie (HOH)

Sequence modifications

A: 39 - 404 (UniProt: P0A988)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Gly 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Gly 14	-	expression tag
Gly 15	-	expression tag
Asn 16	-	expression tag
Asn 17	-	expression tag
Asn 18	-	expression tag
Asn 19	-	expression tag
Asn 20	-	expression tag
Asn 21	-	expression tag
Asn 22	-	expression tag
Asn 23	-	expression tag
Asn 24	-	expression tag
Asn 25	-	expression tag
Leu 26	-	expression tag
Gly 27	-	expression tag
Ile 28	-	expression tag
Glu 29	-	expression tag
Glu 30	-	expression tag
Asn 31	-	expression tag
Leu 32	-	expression tag
Tyr 33	-	expression tag
Phe 34	-	expression tag
Gln 35	-	expression tag
Ser 36	-	expression tag
His 37	-	expression tag
Met 38	-	expression tag
Val 405	-	linker
Asp 406	-	linker
Ser 407	-	linker
Gly 408	-	linker
Ala 409	-	linker
Ser 410	-	linker
Gly 411	-	linker
Gly 412	-	linker
Ser 413	-	linker

A: 414 - 517 (UniProt: A7ZV39)
B: 39 - 404 (UniProt: P0A988)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Gly 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Gly 14	-	expression tag
Gly 15	-	expression tag
Asn 16	-	expression tag
Asn 17	-	expression tag
Asn 18	-	expression tag
Asn 19	-	expression tag
Asn 20	-	expression tag
Asn 21	-	expression tag
Asn 22	-	expression tag
Asn 23	-	expression tag
Asn 24	-	expression tag
Asn 25	-	expression tag
Leu 26	-	expression tag
Gly 27	-	expression tag
Ile 28	-	expression tag
Glu 29	-	expression tag
Glu 30	-	expression tag
Asn 31	-	expression tag
Leu 32	-	expression tag
Tyr 33	-	expression tag
Phe 34	-	expression tag
Gln 35	-	expression tag
Ser 36	-	expression tag
His 37	-	expression tag
Met 38	-	expression tag
Val 405	-	linker
Asp 406	-	linker
Ser 407	-	linker
Gly 408	-	linker
Ala 409	-	linker
Ser 410	-	linker
Gly 411	-	linker
Gly 412	-	linker
Ser 413	-	linker

B: 414 - 517 (UniProt: A7ZV39)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	114181.6
Non-Polymers*	Number of molecules	11
	Total formula weight	1032.9
All*	Total formula weight	115214.5

*Water molecules are not included.