6E8E

Crystal structure of the Escherichia coli sliding clamp-MutL complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97951
Spacegroup name	P 21 21 21
Unit cell lengths	78.390, 103.210, 141.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.830 - 2.250
R-factor	0.191
Rwork	0.190
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x9z 1plq
RMSD bond length	0.004
RMSD bond angle	0.959
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.830	2.320
High resolution limit [Å]	2.070	2.250
Number of reflections	53370
<I/σ(I)>	11.5
Completeness [%]	97.5
Redundancy	4.4
CC(1/2)	0.998	0.356

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM Bis-Tris pH 5.5, 2 M ammonium sulfate.