6E7I

Human ppGalNAcT2 I253A/L310A Mutant with EA2 and UDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polypeptide N-acetylgalactosaminyltransferase 2	polymer	535	60670.1	1	UniProt (Q10471) Pfam (PF00535) Pfam (PF00652)	Homo sapiens (Human)	Polypeptide GalNAc transferase 2,pp-GaNTase 2,Protein-UDP acetylgalactosaminyltransferase 2,UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2
2	B (P)	EA2	polymer	13	1318.4	1	UniProt (Q62605)	Rattus norvegicus
3	C (A)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	1	Chemie (UDP)
4	D (A)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
5	E, F (A, P)	water	water		18.0	327	Chemie (HOH)

Sequence modifications

A: 74 - 571 (UniProt: Q10471)

PDB	External Database	Details
Met 44	-	initiating methionine
Gly 45	-	expression tag
Ile 46	-	expression tag
Leu 47	-	expression tag
Pro 48	-	expression tag
Ser 49	-	expression tag
Pro 50	-	expression tag
Gly 51	-	expression tag
Met 52	-	expression tag
Pro 53	-	expression tag
Ala 54	-	expression tag
Leu 55	-	expression tag
Leu 56	-	expression tag
Ser 57	-	expression tag
Leu 58	-	expression tag
Val 59	-	expression tag
Ser 60	-	expression tag
Leu 61	-	expression tag
Leu 62	-	expression tag
Ser 63	-	expression tag
Val 64	-	expression tag
Leu 65	-	expression tag
Leu 66	-	expression tag
Met 67	-	expression tag
Gly 68	-	expression tag
Cys 69	-	expression tag
Val 70	-	expression tag
Ala 71	-	expression tag
Glu 72	-	expression tag
Thr 73	-	expression tag
Ala 253	Ile 253	engineered mutation
Ala 310	Leu 310	engineered mutation
Lys 572	-	expression tag
His 573	-	expression tag
His 574	-	expression tag
His 575	-	expression tag
His 576	-	expression tag
His 577	-	expression tag
His 578	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61988.5
Non-Polymers*	Number of molecules	2
	Total formula weight	459.1
All*	Total formula weight	62447.6

*Water molecules are not included.