6DPH
Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mn2+ and 200 mM K+ for 120 s at 21 C
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Ribonuclease H | polymer | 142 | 16272.4 | 1 | UniProt (Q9KEI9) In PDB | Bacillus halodurans | RNase H |
| 2 | B | 5'-R(*AP*CP*AP*U)-3' portion of intact RNA (5'-R(*AP*CP*AP*UP*CP*G)-3') | polymer | 4 | 1224.8 | 1 | synthetic construct | ||
| 3 | b | 5'-R(P*CP*G)-3' portion of intact RNA (5'-R(*AP*CP*AP*UP*CP*G)-3') | polymer | 2 | 605.4 | 1 | synthetic construct | ||
| 4 | C | DNA (5'-D(*CP*GP*AP*TP*GP*T)-3') | polymer | 6 | 1824.2 | 1 | synthetic construct | ||
| 5 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | A, C | POTASSIUM ION | non-polymer | 39.1 | 3 | Chemie (K) | |||
| 7 | A | IODIDE ION | non-polymer | 126.9 | 4 | Chemie (IOD) | |||
| 8 | A | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 9 | A | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 10 | A, C | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 11 | water | water | 18.0 | 202 | Chemie (HOH) |
Sequence modifications
A: 59 - 196 (UniProt: Q9KEI9)
| PDB | External Database | Details |
|---|---|---|
| Gly 55 | - | expression tag |
| Ser 56 | - | expression tag |
| His 57 | - | expression tag |
| Met 58 | - | expression tag |
| Ala 188 | Glu 188 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 19926.9 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 1378.3 | |
| All* | Total formula weight | 21305.2 |
