6DPH
Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mn2+ and 200 mM K+ for 120 s at 21 C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.867, 37.063, 61.789 |
| Unit cell angles | 90.00, 96.33, 90.00 |
Refinement procedure
| Resolution | 23.197 - 1.339 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.19180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 1ZBL |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.066 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 23.200 | 23.200 | 1.360 |
| High resolution limit [Å] | 1.339 | 7.330 | 1.340 |
| Number of reflections | 40256 | 241 | 1339 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 97.4 | 88.2 | 66.1 |
| Redundancy | 1 | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |
