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6DOV

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 80 s at 21 C

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
Ribonuclease Hpolymer13615716.71UniProt (Q9KEI9)Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)RNase H
2B
(B)
5'-R(*AP*CP*AP*U)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3'polymer41224.81synthetic construct
3C
(b)
5'-R(P*CP*G)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3'polymer2605.41synthetic construct
4D
(C)
DNA (5'-D(*CP*GP*AP*TP*GP*T)-3')polymer61824.21synthetic construct
5E, F
(A)
MAGNESIUM IONnon-polymer24.32Chemie (MG)
6G, H, I, J, R
(A, C)
RUBIDIUM IONnon-polymer85.55Chemie (RB)
7K, L, M
(A)
CHLORIDE IONnon-polymer35.53Chemie (CL)
8N, O, P, S
(A, C)
GLYCEROLnon-polymer92.14Chemie (GOL)
9Q
(A)
1,2-ETHANEDIOLnon-polymer62.11Chemie (EDO)
10T, U, V, W
(A, B, b, C)
waterwater18.0207Chemie (HOH)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight19371.2
Non-Polymers*Number of molecules15
Total formula weight1012.8
All*Total formula weight20383.9
*Water molecules are not included.

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PDB entries from 2024-11-06

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