6D8F

RsAgo Ternary Complex with Guide RNA and Target DNA Containing T-T Bulge Within the Seed Segment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, F)	Uncharacterized protein	polymer	791	89038.4	2	UniProt (A4WYU7) Pfam (PF02171)	Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
2	B, E (C, H)	RNA (5'-R(P*UP*UP*AP*CP*UP*GP*CP*AP*CP*AP*GP*GP*UP*GP*AP*CP*GP*A)-3')	polymer	18	5772.5	2		Rhodobacter sphaeroides ATCC 17025
3	C, F (G, J)	DNA (5'-D(P*TP*CP*GP*TP*CP*AP*CP*CP*TP*GP*TP*GP*CP*AP*GP*TP*TP*TP*AP*AP*C)-3')	polymer	26	7945.1	2		Rhodobacter sphaeroides ATCC 17025
4	G, H, K, L, O (A, F, J)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	5	Chemie (MPD)
5	I (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
6	J, M, N (C, H, J)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
7	P, Q, R, S, T... (A, C, G, F, H...)	water	water		18.0	642	Chemie (HOH)

Sequence modifications

A, F: 2 - 777 (UniProt: A4WYU7)

PDB	External Database	Details
Met -13	-	initiating methionine
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	205512.0
Non-Polymers*	Number of molecules	9
	Total formula weight	722.8
All*	Total formula weight	206234.8

*Water molecules are not included.