6D8F

RsAgo Ternary Complex with Guide RNA and Target DNA Containing T-T Bulge Within the Seed Segment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97921
Spacegroup name	P 1 21 1
Unit cell lengths	68.039, 118.312, 117.672
Unit cell angles	90.00, 95.48, 90.00

Refinement procedure

Resolution	42.300 - 2.150
Rwork	0.201
R-free	0.24000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.095	0.637
Number of reflections	100379
<I/σ(I)>	12.7	2.2
Completeness [%]	99.9	100
Redundancy	3.8	3.8
CC(1/2)	0.992	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	40% MPD, 30 mM magnesium acetate, 50 mM sodium cacodylate pH 5.7