6D65
Crystal structure of the human dual specificity phosphatase 1 catalytic domain (C258S) as a maltose binding protein fusion in complex with the designed AR protein off7
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | Maltose-binding periplasmic protein,Dual specificity protein phosphatase 1 | polymer | 520 | 57103.6 | 2 | UniProt (P0AEX9) UniProt (P28562) Pfam (PF01547) Pfam (PF00782) In PDB | Escherichia coli (strain K12) | MBP,MMBP,Maltodextrin-binding protein,Dual specificity protein phosphatase hVH1,Mitogen-activated protein kinase phosphatase 1,MKP-1,Protein-tyrosine phosphatase CL100 |
2 | B, D | Designed AR protein off7 | polymer | 169 | 18308.5 | 2 | synthetic construct | ||
3 | A, C | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
4 | C, A, B, D | SULFATE ION | non-polymer | 96.1 | 23 | Chemie (SO4) | |||
5 | B, D, C, A | ETHANOL | non-polymer | 46.1 | 21 | Chemie (EOH) | |||
6 | water | water | 18.0 | 606 | Chemie (HOH) |
Sequence modifications
A, C: 2 - 366 (UniProt: P0AEX9)
A, C: 372 - 514 (UniProt: P28562)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
Ala 83 | Asp 108 | engineered mutation |
Ala 84 | Lys 109 | engineered mutation |
Ala 173 | Glu 198 | engineered mutation |
Ala 174 | Asn 199 | engineered mutation |
Ala 240 | Lys 265 | engineered mutation |
Ala 360 | Glu 385 | engineered mutation |
Ala 363 | Lys 388 | engineered mutation |
Ala 364 | Asp 389 | engineered mutation |
Thr 367 | - | linker |
Asn 368 | - | linker |
Ala 369 | - | linker |
Ala 370 | - | linker |
Ala 371 | - | linker |
PDB | External Database | Details |
---|---|---|
Ser 458 | Cys 258 | engineered mutation |
His 515 | - | expression tag |
His 516 | - | expression tag |
His 517 | - | expression tag |
His 518 | - | expression tag |
His 519 | - | expression tag |
His 520 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 150824.2 | |
Non-Polymers* | Number of molecules | 50 |
Total formula weight | 3729.4 | |
All* | Total formula weight | 154553.6 |