6CVL

Crystal structure of the Escherichia coli ATPgS-bound MetNI methionine ABC transporter in complex with its MetQ binding protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, D	MetN nucleotide-binding subunit	polymer	344	37925.4	2	UniProt (P30750) Pfam (PF00005) Pfam (PF09383) In PDB	Escherichia coli (strain K12)
2	A, B	MetI transmembrane subunit	polymer	215	22983.6	2	UniProt (P31547) Pfam (PF00528) In PDB	Escherichia coli (strain K12)
3	E	MetQ periplasmic binding protein	polymer	226	24724.1	1	UniProt (P28635) Pfam (PF03180) In PDB	Escherichia coli (strain K12)
4	C, D	MERCURY (II) ION	non-polymer		200.6	3	Chemie (HG)
5	C, D	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	2	Chemie (AGS)
6	D	IODIDE ION	non-polymer		126.9	2	Chemie (IOD)

Sequence modifications

C, D: 1 - 343 (UniProt: P30750)

PDB	External Database	Details
His 0	-	expression tag
Gln 166	Glu 166	engineered mutation
Ala 295	Asn 295	engineered mutation

E: 34 - 259 (UniProt: P28635)

PDB	External Database	Details
Ala 229	Asn 229	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	146542.1
Non-Polymers*	Number of molecules	7
	Total formula weight	1902.1
All*	Total formula weight	148444.1

*Water molecules are not included.