6CVL
Crystal structure of the Escherichia coli ATPgS-bound MetNI methionine ABC transporter in complex with its MetQ binding protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (C, D) | MetN nucleotide-binding subunit | polymer | 344 | 37925.4 | 2 | UniProt (P30750) Pfam (PF00005) Pfam (PF09383) | Escherichia coli (strain K12) | |
| 2 | B, C (A, B) | MetI transmembrane subunit | polymer | 215 | 22983.6 | 2 | UniProt (P31547) Pfam (PF00528) | Escherichia coli (strain K12) | |
| 3 | E (E) | MetQ periplasmic binding protein | polymer | 226 | 24724.1 | 1 | UniProt (P28635) Pfam (PF03180) | Escherichia coli (strain K12) | |
| 4 | F, G, I (C, D) | MERCURY (II) ION | non-polymer | 200.6 | 3 | Chemie (HG) | |||
| 5 | H, L (C, D) | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 2 | Chemie (AGS) | |||
| 6 | J, K (D) | IODIDE ION | non-polymer | 126.9 | 2 | Chemie (IOD) |
Sequence modifications
C, D: 1 - 343 (UniProt: P30750)
E: 34 - 259 (UniProt: P28635)
| PDB | External Database | Details |
|---|---|---|
| His 0 | - | expression tag |
| Gln 166 | Glu 166 | engineered mutation |
| Ala 295 | Asn 295 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 229 | Asn 229 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 146542.1 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1902.1 | |
| All* | Total formula weight | 148444.1 |
