6CVL

Crystal structure of the Escherichia coli ATPgS-bound MetNI methionine ABC transporter in complex with its MetQ binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00582
Spacegroup name	P 32 2 1
Unit cell lengths	107.960, 107.960, 354.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.853 - 2.953
R-factor	0.2111
Rwork	0.208
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.853	39.853	3.030
High resolution limit [Å]	2.950	13.210	2.950
Rmerge	0.129	0.064	1.874
Rmeas	0.133	0.066	1.933
Rpim	0.031
Total number of observations	821267
Number of reflections	43138	666	295
<I/σ(I)>	14.64	36.69	1.45
Completeness [%]	83.7	94.2	7.9
Redundancy	19.038	15.188	16.549
CC(1/2)	0.999	0.998	0.472

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	22% PEG 400, 0.1M MES pH 6.0