6CUU

Thermus thermophiles RNA polymerase in complex with promoter DNA and antibiotic Kanglemycin A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	315	35056.2	2	UniProt (Q72I32) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (Q5SHR6)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1119	125436.5	1	UniProt (Q72HM5) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (Q8RQE9)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1524	170997.4	1	UniProt (Q72HM6) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (Q8RQE8)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	99	11533.3	1	UniProt (Q72ID6) Pfam (PF01192) UniProt (by SIFTS) (Q8RQE7)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	RNA polymerase sigma factor SigA	polymer	423	48568.0	1	UniProt (Q72L95) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (Q5SKW1)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
6	G (G)	DNA (5'-D(P*TP*GP*CP*AP*TP*CP*AP*GP*AP*GP*CP*CP*CP*AP*AP*AP*A)-3')	polymer	22	6674.3	1		Bacillus subtilis
7	H (H)	DNA (5'-D(*TP*AP*TP*AP*AP*TP*GP*GP*GP*AP*GP*CP*CP*TP*CP*TP*GP*AP*TP*GP*CP*A)-3')	polymer	27	8412.4	1		Bacillus subtilis
8	I (C)	Kanglemycin A	non-polymer		986.1	1	Chemie (KNG)
9	J, K (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	L, M (D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

C: 1 - 1119 (UniProt: Q72HM5)

PDB	External Database	Details
Thr 958	Pro 958	conflict

D: 1 - 1524 (UniProt: Q72HM6)

PDB	External Database	Details
Arg 274	Gln 274	conflict
Leu 1041	Met 1041	conflict
Val 1313	Ala 1313	conflict

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	441734.3
Non-Polymers*	Number of molecules	5
	Total formula weight	1165.5
All*	Total formula weight	442899.8

*Water molecules are not included.