6CUU
Thermus thermophiles RNA polymerase in complex with promoter DNA and antibiotic Kanglemycin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.221, 101.315, 294.279 |
| Unit cell angles | 90.00, 98.89, 90.00 |
Refinement procedure
| Resolution | 46.305 - 2.994 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.089 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.304 | 2.864 |
| High resolution limit [Å] | 2.794 | 2.794 |
| Number of reflections | 127584 | |
| <I/σ(I)> | 7.78 | |
| Completeness [%] | 95.5 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M Tris-HCl (pH 8.0), 0.2 M KCl, 50 mM MgCl2, 9.5% PEG4000 |
