6CRK

Heterotrimeric G-protein in complex with an antibody fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	355	40438.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37728.2	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (H)	single-chain Fv	polymer	259	27784.9	1		Mus musculus (mouse)
5	E (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
6	F (A)	CITRATE ANION	non-polymer		189.1	1	Chemie (FLC)
7	G, H, I, J (A, B, G, H)	water	water		18.0	627	Chemie (HOH)

Sequence modifications

A: 2 - 354 (UniProt: P63096)

PDB	External Database	Details
Gly 0	-	expression tag
Pro 1	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

G: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	113796.1
Non-Polymers*	Number of molecules	2
	Total formula weight	632.3
All*	Total formula weight	114428.4

*Water molecules are not included.