6CRK

Heterotrimeric G-protein in complex with an antibody fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 2 2 21
Unit cell lengths	58.514, 104.739, 211.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.260 - 2.000
Rwork	0.175
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1GP2 and 4NKD
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.260	39.260	2.040
High resolution limit [Å]	2.000	10.770	2.000
Rmerge	0.061	0.026	1.084
Rmeas	0.069	0.030	1.225
Rpim	0.031	0.014	0.556
Total number of observations	409653	2790	21119
Number of reflections	88263	651	4462
<I/σ(I)>	13.5	47.7	1.5
Completeness [%]	99.4	96.5	99.5
Redundancy	4.6	4.3	4.7
CC(1/2)	0.998	0.994	0.474

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	10% PEG 8000, 0.1M Sodium citrate pH 5.0, 1mM MgCl2, 10uM GDP, 100uM TCEP, 1mM aluminium chloride, 10mM sodium fluoride