6CRI
Structure of the cargo bound AP-1:Arf1:tetherin-Nef stable closed trimer
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | T, N, c, Y, d... | Bone marrow stromal antigen 2, Protein Nef chimera | polymer | 264 | 29964.3 | 6 | UniProt (Q10589) UniProt (Q90VU7) Pfam (PF00469) UniProt (by SIFTS) (P03404) In PDB | Homo sapiens (Human) | BST-2, HM1.24 antigen, Tetherin |
2 | B, I, J | AP-1 complex subunit beta-1 | polymer | 570 | 64458.7 | 3 | UniProt (Q10567) Pfam (PF01602) In PDB | Homo sapiens (Human) | Adaptor protein complex AP-1 subunit beta-1, Adaptor-related protein complex 1 subunit beta-1, Beta-1-adaptin, Beta-adaptin 1, Clathrin assembly protein complex 1 beta large chain, Golgi adaptor HA1/AP1 adaptin beta subunit |
3 | C, H, K, U, L... | ADP-ribosylation factor 1 | polymer | 165 | 18936.6 | 6 | UniProt (P84077) Pfam (PF00025) In PDB | Homo sapiens (Human) | Arf1 |
4 | G, Q, R | AP-1 complex subunit gamma-1 | polymer | 585 | 66401.1 | 3 | UniProt (P22892) Pfam (PF01602) In PDB | Mus musculus (Mouse) | Adaptor protein complex AP-1 subunit gamma-1, Adaptor-related protein complex 1 subunit gamma-1, Clathrin assembly protein complex 1 gamma-1 large chain, Gamma-adaptin, Gamma1-adaptin, Golgi adaptor HA1/AP1 adaptin subunit gamma-1 |
5 | M, W, X | AP-1 complex subunit mu-1 | polymer | 422 | 48475.5 | 3 | UniProt (P35585) Pfam (PF01217) Pfam (PF00928) In PDB | Mus musculus (Mouse) | AP-mu chain family member mu1A, Adaptor protein complex AP-1 subunit mu-1, Adaptor-related protein complex 1 subunit mu-1, Clathrin assembly protein complex 1 mu-1 medium chain 1, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, Mu-adaptin 1, Mu1A-adaptin |
6 | S, a, b | AP-1 complex subunit sigma-3 | polymer | 142 | 16998.8 | 3 | UniProt (Q96PC3) Pfam (PF01217) In PDB | Homo sapiens (Human) | Adaptor protein complex AP-1 subunit sigma-1C, Adaptor-related protein complex 1 subunit sigma-1C, Clathrin assembly protein complex 1 sigma-1C small chain, Golgi adaptor HA1/AP1 adaptin sigma-1C subunit, Sigma 1C subunit of AP-1 clathrin, Sigma-adaptin 1C, Sigma1C-adaptin |
7 | H, K, U, L, V... | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 6 | Chemie (GTP) | |||
8 | H, K, U, L, V... | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) |
Sequence modifications
T, N, c, Y, d, Z: 2 - 21 (UniProt: Q10589)
T, N, c, Y, d, Z: 32 - 237 (UniProt: Q90VU7)
B, I, J: 14 - 583 (UniProt: Q10567)
C, H, K, U, L, V: 17 - 181 (UniProt: P84077)
PDB | External Database | Details |
---|---|---|
Met -26 | - | expression tag |
Ser -25 | - | expression tag |
Tyr -24 | - | expression tag |
Tyr -23 | - | expression tag |
His -22 | - | expression tag |
His -21 | - | expression tag |
His -20 | - | expression tag |
His -19 | - | expression tag |
His -18 | - | expression tag |
His -17 | - | expression tag |
Asp -16 | - | expression tag |
Tyr -15 | - | expression tag |
Asp -14 | - | expression tag |
Ile -13 | - | expression tag |
Pro -12 | - | expression tag |
Thr -11 | - | expression tag |
Thr -10 | - | expression tag |
Glu -9 | - | expression tag |
Asn -8 | - | expression tag |
Leu -7 | - | expression tag |
Tyr -6 | - | expression tag |
Phe -5 | - | expression tag |
Gln -4 | - | expression tag |
Gly -3 | - | expression tag |
Ala -2 | - | expression tag |
Met -1 | - | expression tag |
Gly 0 | - | expression tag |
Ser 1 | - | expression tag |
Gly 22 | - | linker |
Ser 23 | - | linker |
Asp 24 | - | linker |
Glu 25 | - | linker |
Ala 26 | - | linker |
Ser 27 | - | linker |
Glu 28 | - | linker |
Gly 29 | - | linker |
Ser 30 | - | linker |
Gly 31 | - | linker |
B, I, J: 14 - 583 (UniProt: Q10567)
PDB | External Database | Details |
---|---|---|
Arg 359 | Lys 359 | engineered mutation |
Lys 476 | Glu 476 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Leu 71 | Gln 71 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 24 |
Total formula weight | 882407.8 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 3284.9 | |
All* | Total formula weight | 885692.8 |