6CRI

Structure of the cargo bound AP-1:Arf1:tetherin-Nef stable closed trimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, H, I, P, Q... (T, N, c, Y, d...)	Bone marrow stromal antigen 2, Protein Nef chimera	polymer	264	29964.3	6	UniProt (Q10589) UniProt (Q90VU7) UniProt (by SIFTS) (P03404)	Homo sapiens (Human)	BST-2, HM1.24 antigen, Tetherin
2	B, J, R (B, I, J)	AP-1 complex subunit beta-1	polymer	570	64458.7	3	UniProt (Q10567) Pfam (PF01602)	Homo sapiens (Human)	Adaptor protein complex AP-1 subunit beta-1, Adaptor-related protein complex 1 subunit beta-1, Beta-1-adaptin, Beta-adaptin 1, Clathrin assembly protein complex 1 beta large chain, Golgi adaptor HA1/AP1 adaptin beta subunit
3	C, E, K, M, S... (C, H, K, U, L...)	ADP-ribosylation factor 1	polymer	165	18936.6	6	UniProt (P84077) Pfam (PF00025)	Homo sapiens (Human)	Arf1
4	D, L, T (G, Q, R)	AP-1 complex subunit gamma-1	polymer	585	66401.1	3	UniProt (P22892) Pfam (PF01602)	Mus musculus (Mouse)	Adaptor protein complex AP-1 subunit gamma-1, Adaptor-related protein complex 1 subunit gamma-1, Clathrin assembly protein complex 1 gamma-1 large chain, Gamma-adaptin, Gamma1-adaptin, Golgi adaptor HA1/AP1 adaptin subunit gamma-1
5	F, N, V (M, W, X)	AP-1 complex subunit mu-1	polymer	422	48475.5	3	UniProt (P35585) Pfam (PF01217) Pfam (PF00928)	Mus musculus (Mouse)	AP-mu chain family member mu1A, Adaptor protein complex AP-1 subunit mu-1, Adaptor-related protein complex 1 subunit mu-1, Clathrin assembly protein complex 1 mu-1 medium chain 1, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, Mu-adaptin 1, Mu1A-adaptin
6	G, O, W (S, a, b)	AP-1 complex subunit sigma-3	polymer	142	16998.8	3	UniProt (Q96PC3) Pfam (PF01217)	Homo sapiens (Human)	Adaptor protein complex AP-1 subunit sigma-1C, Adaptor-related protein complex 1 subunit sigma-1C, Clathrin assembly protein complex 1 sigma-1C small chain, Golgi adaptor HA1/AP1 adaptin sigma-1C subunit, Sigma 1C subunit of AP-1 clathrin, Sigma-adaptin 1C, Sigma1C-adaptin
7	AA, CA, EA, GA, IA... (H, K, U, L, V...)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	6	Chemie (GTP)
8	BA, DA, FA, HA, JA... (H, K, U, L, V...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)

Sequence modifications

T, N, c, Y, d, Z: 2 - 21 (UniProt: Q10589)

PDB	External Database	Details
Met -26	-	expression tag
Ser -25	-	expression tag
Tyr -24	-	expression tag
Tyr -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Asp -14	-	expression tag
Ile -13	-	expression tag
Pro -12	-	expression tag
Thr -11	-	expression tag
Thr -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Gly 22	-	linker
Ser 23	-	linker
Asp 24	-	linker
Glu 25	-	linker
Ala 26	-	linker
Ser 27	-	linker
Glu 28	-	linker
Gly 29	-	linker
Ser 30	-	linker
Gly 31	-	linker

T, N, c, Y, d, Z: 32 - 237 (UniProt: Q90VU7)
B, I, J: 14 - 583 (UniProt: Q10567)

PDB	External Database	Details
Arg 359	Lys 359	engineered mutation
Lys 476	Glu 476	engineered mutation

C, H, K, U, L, V: 17 - 181 (UniProt: P84077)

PDB	External Database	Details
Leu 71	Gln 71	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	882407.8
Non-Polymers*	Number of molecules	12
	Total formula weight	3284.9
All*	Total formula weight	885692.8

*Water molecules are not included.