6CER

Human pyruvate dehydrogenase complex E1 component V138M mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial	polymer	365	40746.6	4	UniProt (P08559) Pfam (PF00676) In PDB	Homo sapiens (Human)	PDHE1-A type I
2	B, D, F, H	Pyruvate dehydrogenase E1 component subunit beta, mitochondrial	polymer	331	36085.4	4	UniProt (P11177) Pfam (PF02779) Pfam (PF02780) In PDB	Homo sapiens (Human)	PDHE1-B
3	A, C, E, G	THIAMINE DIPHOSPHATE	non-polymer		425.3	4	Chemie (TPP)
4	E, G	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5		water	water		18.0	302	Chemie (HOH)

Sequence modifications

A, C, E, G: 1 - 361 (UniProt: P08559)

PDB	External Database	Details
Met -3	-	initiating methionine
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Met 138	Val 167	engineered mutation

B, D, F, H: 1 - 329 (UniProt: P11177)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	307328.0
Non-Polymers*	Number of molecules	6
	Total formula weight	1749.9
All*	Total formula weight	309077.8

*Water molecules are not included.