6BS1

Crystal Structure of Human DNA polymerase kappa in complex with DNA containing the major cisplatin lesion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA polymerase kappa	polymer	551	63287.1	2	UniProt (Q9UBT6) Pfam (PF00817) Pfam (PF11798) Pfam (PF11799) In PDB	Homo sapiens (Human)	DINB protein,DINP
2	C, P	DNA (5'-D(P*AP*TP*AP*CP*AP*TP*AP*CP*C)-3')	polymer	9	2683.8	2		synthetic construct
3	D, T	DNA (5'-D(*TP*AP*CP*TP*GP*GP*TP*AP*TP*GP*TP*AP*T)-3')	polymer	13	3996.6	2		synthetic construct
4	A, B	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
5	A, B	IODIDE ION	non-polymer		126.9	9	Chemie (IOD)
6	A, B	2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]adenosine	non-polymer		490.2	2	Chemie (DZ4)
7	D, T	Cisplatin	non-polymer		300.0	2	Chemie (CPT)
8		water	water		18.0	25	Chemie (HOH)

Sequence modifications

A, B: 1 - 526 (UniProt: Q9UBT6)

PDB	External Database	Details
Met -24	-	initiating methionine
Ser -23	-	expression tag
Tyr -22	-	expression tag
Tyr -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	139935.0
Non-Polymers*	Number of molecules	19
	Total formula weight	2868.4
All*	Total formula weight	142803.4

*Water molecules are not included.