6BS1
Crystal Structure of Human DNA polymerase kappa in complex with DNA containing the major cisplatin lesion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-14 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9801 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.800, 127.720, 168.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.270 - 3.150 |
R-factor | 0.24705 |
Rwork | 0.244 |
R-free | 0.29851 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.332 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.270 | 3.320 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.101 | 0.550 |
Number of reflections | 23000 | |
<I/σ(I)> | 8 | |
Completeness [%] | 93.7 | |
Redundancy | 3.6 | |
CC(1/2) | 0.996 | 0.791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 20-25% PEG5KMME and 0.1-0.2M ammonium iodide |