6BKD

Structure of Hepatitis C Virus Envelope Glycoprotein E2 core from genotype 6a bound to broadly neutralizing antibody AR3D

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (E)	Polyprotein	polymer	189	20707.4	1	UniProt (B9V0E2) Pfam (PF01560)	Recombinant Hepatitis C virus HK6a/JFH-1
2	B (H)	Fab AR3D heavy chain	polymer	234	24458.6	1	UniProt (S6B2A6) Pfam (PF07686) Pfam (PF07654)	Homo sapiens (Human)
3	C (L)	Fab AR3D light chain	polymer	212	23047.5	1	UniProt (Q6PIL8) Pfam (PF07686) Pfam (PF07654)	Homo sapiens (Human)
4	D (A)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)

Sequence modifications

E: 412 - 481 (UniProt: B9V0E2)

PDB	External Database	Details
Asp 470	Asn 448	engineered mutation

E: 486 - 568 (UniProt: B9V0E2)

PDB	External Database	Details
Gly 482	-	linker
Ser 483	-	linker
Ser 484	-	linker
Gly 485	-	linker

E: 598 - 645 (UniProt: B9V0E2)

PDB	External Database	Details
Gly 592	-	linker
Gly 593	-	linker
Pro 594	-	linker
Thr 595	-	linker
Asp 596	-	linker
Gly 597	-	linker

H: 3 - 102 (PDB: 6BKD)
H: 103 - 216 (UniProt: S6B2A6)

PDB	External Database	Details
Leu 108	Thr 139	conflict
Lys 210	Arg 241	conflict
Gly 217	-	expression tag
Ser 218	-	expression tag

L: 3 - 96 (PDB: 6BKD)
L: 97 - 214 (UniProt: Q6PIL8)

PDB	External Database	Details
Glu 105	Asp 127	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	68213.5
Branched	Number of molecules	1
	Total formula weight	424.4
All*	Total formula weight	68637.9

*Water molecules are not included.