6BKD
Structure of Hepatitis C Virus Envelope Glycoprotein E2 core from genotype 6a bound to broadly neutralizing antibody AR3D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0331 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.318, 60.392, 148.082 |
| Unit cell angles | 90.00, 88.00, 90.00 |
Refinement procedure
| Resolution | 49.330 - 3.250 |
| R-factor | 0.2698 |
| Rwork | 0.268 |
| R-free | 0.28610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bkb |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.835 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.250 | 3.300 |
| Rpim | 0.070 | |
| Number of reflections | 9230 | |
| <I/σ(I)> | 7.5 | 1.2 |
| Completeness [%] | 90.1 | 68.3 |
| Redundancy | 8.1 | 8.1 |
| CC(1/2) | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 20% (w/v) PEG 6000, 0.1M Hepes, pH 7.0 |
