6BC8

Crystal structure of Rev7-R124A/Rev3-RBM2 (residues 1988-2014) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Mitotic spindle assembly checkpoint protein MAD2B	polymer	227	26101.2	1	UniProt (Q9UI95) Pfam (PF02301)	Homo sapiens (Human)	Mitotic arrest deficient 2-like protein 2,MAD2-like protein 2,REV7 homolog,hREV7
2	B (B)	DNA polymerase zeta catalytic subunit	polymer	28	3278.0	1	UniProt (O60673)	Homo sapiens (Human)	Protein reversionless 3-like,hREV3
3	C, D (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	E, F (A)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
5	G, H (A, B)	water	water		18.0	140	Chemie (HOH)

Sequence modifications

A: 1 - 211 (UniProt: Q9UI95)

PDB	External Database	Details
Met -15	-	initiating methionine
Gly -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Ser -5	-	expression tag
Gln -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Asn -1	-	expression tag
Ser 0	-	expression tag
Ala 124	Arg 124	engineered mutation

B: 1988 - 2014 (UniProt: O60673)

PDB	External Database	Details
Met 1987	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	29379.2
Non-Polymers*	Number of molecules	4
	Total formula weight	310.2
All*	Total formula weight	29689.5

*Water molecules are not included.