6BC8
Crystal structure of Rev7-R124A/Rev3-RBM2 (residues 1988-2014) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 77.15 |
| Detector technology | PIXEL |
| Collection date | 2016-03-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 63.691, 63.691, 116.274 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 116.270 - 1.680 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ABD (Rev7 molecule only) |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.840 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 116.270 |
| High resolution limit [Å] | 1.680 |
| Rmerge | 0.063 |
| Rmeas | 0.067 |
| Rpim | 0.022 |
| Number of reflections | 31583 |
| <I/σ(I)> | 40.4 |
| Completeness [%] | 99.6 |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292.15 | well solution containing 100 mM sodium acetate, 200 mM NaCl, 1.4 M ammonium sulfate was mixed at 1:1 ratio with protein at 45 mg/mL in 5 mM HEPES, 100 mM NaCl, 10 mM DTT, pH=7.4 |
