6B5B

Cryo-EM structure of the NAIP5-NLRC4-flagellin inflammasome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Baculoviral IAP repeat-containing protein 1e	polymer	1403	159874.8	1	UniProt (Q9R016) Pfam (PF00653) Pfam (PF05729) Pfam (PF22524) Pfam (PF17889) In PDB	Mus musculus (Mouse)	Neuronal apoptosis inhibitory protein 5
2	B, C	NLR family CARD domain-containing protein 4	polymer	1024	116886.0	2	UniProt (Q3UP24) Pfam (PF17889) Pfam (PF00619) Pfam (PF05729) Pfam (PF22524) In PDB	Mus musculus (Mouse)	Caspase recruitment domain-containing protein 12, Ice protease-activating factor, Ipaf
3	F	Flagellin	polymer	566	58494.1	1	UniProt (G8UUW9) Pfam (PF00669) Pfam (PF07196) Pfam (PF00700) UniProt (by SIFTS) (Q5ZVV0) In PDB	Legionella pneumophila

Sequence modifications

F: 2 - 475 (UniProt: G8UUW9)

PDB	External Database	Details
Met -90	-	initiating methionine
Glu -89	-	expression tag
Gln -88	-	expression tag
Lys -87	-	expression tag
Leu -86	-	expression tag
Ile -85	-	expression tag
Ser -84	-	expression tag
Glu -83	-	expression tag
Glu -82	-	expression tag
Asp -81	-	expression tag
Leu -80	-	expression tag
Asn -79	-	expression tag
Glu -78	-	expression tag
Met -77	-	expression tag
Glu -76	-	expression tag
Gln -75	-	expression tag
Lys -74	-	expression tag
Leu -73	-	expression tag
Ile -72	-	expression tag
Ser -71	-	expression tag
Glu -70	-	expression tag
Glu -69	-	expression tag
Asp -68	-	expression tag
Leu -67	-	expression tag
Asn -66	-	expression tag
Glu -65	-	expression tag
Met -64	-	expression tag
Glu -63	-	expression tag
Gln -62	-	expression tag
Lys -61	-	expression tag
Leu -60	-	expression tag
Ile -59	-	expression tag
Ser -58	-	expression tag
Glu -57	-	expression tag
Glu -56	-	expression tag
Asp -55	-	expression tag
Leu -54	-	expression tag
Asn -53	-	expression tag
Glu -52	-	expression tag
Met -51	-	expression tag
Glu -50	-	expression tag
Gln -49	-	expression tag
Lys -48	-	expression tag
Leu -47	-	expression tag
Ile -46	-	expression tag
Ser -45	-	expression tag
Glu -44	-	expression tag
Glu -43	-	expression tag
Asp -42	-	expression tag
Leu -41	-	expression tag
Asn -40	-	expression tag
Glu -39	-	expression tag
Met -38	-	expression tag
Glu -37	-	expression tag
Gln -36	-	expression tag
Lys -35	-	expression tag
Leu -34	-	expression tag
Ile -33	-	expression tag
Ser -32	-	expression tag
Glu -31	-	expression tag
Glu -30	-	expression tag
Asp -29	-	expression tag
Leu -28	-	expression tag
Asn -27	-	expression tag
Glu -26	-	expression tag
Met -25	-	expression tag
Glu -24	-	expression tag
Ser -23	-	expression tag
Leu -22	-	expression tag
Gly -21	-	expression tag
Asp -20	-	expression tag
Leu -19	-	expression tag
Thr -18	-	expression tag
Met -17	-	expression tag
Glu -16	-	expression tag
Gln -15	-	expression tag
Lys -14	-	expression tag
Leu -13	-	expression tag
Ile -12	-	expression tag
Ser -11	-	expression tag
Glu -10	-	expression tag
Glu -9	-	expression tag
Asp -8	-	expression tag
Leu -7	-	expression tag
Asn -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Arg -3	-	expression tag
Pro -2	-	expression tag
Ala -1	-	expression tag
Ala 0	-	expression tag
Met 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	452140.9
All*	Total formula weight	452140.9

*Water molecules are not included.