6B5B
Cryo-EM structure of the NAIP5-NLRC4-flagellin inflammasome
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.2 |
| Number of Particle | 856358 |
| Software | RELION,EMAN2,PHENIX,I-TASSER,SerialEM,Gctf,UCSF Chimera,ADP_EM,iMODFIT |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
