6B5B
Cryo-EM structure of the NAIP5-NLRC4-flagellin inflammasome
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK II |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 5.2 |
Number of Particle | 856358 |
Software | RELION,EMAN2,PHENIX,I-TASSER,SerialEM,Gctf,UCSF Chimera,ADP_EM,iMODFIT |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |