6B1M

Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Heat shock protein family A (Hsp70) member 8	polymer	400	44134.8	2	UniProt (P11142) Pfam (PF00012)	Homo Sapiens
2	C, E (B, A)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
3	D, F (B, A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	G, H (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	I, J (B, A)	water	water		18.0	854	Chemie (HOH)

Sequence modifications

B, A: 5 - 381 (UniProt: P11142)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Trp 17	Cys 17	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88269.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1245.2
All*	Total formula weight	89514.8

*Water molecules are not included.