6B1M
Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.809, 78.253, 75.855 |
| Unit cell angles | 90.00, 101.42, 90.00 |
Refinement procedure
| Resolution | 40.526 - 1.900 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6b1i |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.742 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.400 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 74694 |
| <I/σ(I)> | 9.5 |
| Completeness [%] | 95.9 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | NaCl, Tris, PEG 3350, MgCl2, AppNHp |
