6AM0
Crystal structure of K. lactis Edc1-Dcp1-Dcp2-Edc3 decapping complex with synthetic cap substrate analog
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, E | KLLA0F23980p | polymer | 275 | 31804.9 | 2 | UniProt (Q6CIU1) Pfam (PF05026) Pfam (PF00293) In PDB | Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) | |
2 | B, F | KLLA0E01827p | polymer | 190 | 22074.0 | 2 | UniProt (Q6CPV9) Pfam (PF06058) In PDB | Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) | |
3 | C, G | KLLA0A01474p | polymer | 26 | 2755.1 | 2 | UniProt (Q6CYC5) In PDB | Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) | |
4 | D, H | KLLA0A11308p | polymer | 66 | 7331.6 | 2 | UniProt (Q6CX48) In PDB | Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) | |
5 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
6 | A, E | [[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-7-methyl-6-oxidanylidene-3~{H}-purin-7-ium-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl] [[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-7-methyl-6-oxidanylidene-3~{H}-purin-7-ium-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl]oxy-oxidanyl-phosphoryl] hydrogen phosphate | non-polymer | 930.6 | 2 | Chemie (6VQ) |
Sequence modifications
A, E: 1 - 275 (UniProt: Q6CIU1)
B, F: 1 - 188 (UniProt: Q6CPV9)
PDB | External Database | Details |
---|---|---|
Gln 152 | Glu 152 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 127931.1 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 1885.5 | |
All* | Total formula weight | 129816.6 |