6AM0

Crystal structure of K. lactis Edc1-Dcp1-Dcp2-Edc3 decapping complex with synthetic cap substrate analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	KLLA0F23980p	polymer	275	31804.9	2	UniProt (Q6CIU1) Pfam (PF05026) Pfam (PF00293)	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast)
2	B, F (B, F)	KLLA0E01827p	polymer	190	22074.0	2	UniProt (Q6CPV9) Pfam (PF06058)	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast)
3	C, G (C, G)	KLLA0A01474p	polymer	26	2755.1	2	UniProt (Q6CYC5)	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
4	D, H (D, H)	KLLA0A11308p	polymer	66	7331.6	2	UniProt (Q6CX48)	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast)
5	I (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	J, K (A, E)	[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-7-methyl-6-oxidanylidene-3~{H}-purin-7-ium-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl] [[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-7-methyl-6-oxidanylidene-3~{H}-purin-7-ium-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl]oxy-oxidanyl-phosphoryl] hydrogen phosphate	non-polymer		930.6	2	Chemie (6VQ)

Sequence modifications

A, E: 1 - 275 (UniProt: Q6CIU1)

PDB	External Database	Details
Gln 152	Glu 152	engineered mutation

B, F: 1 - 188 (UniProt: Q6CPV9)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	127931.1
Non-Polymers*	Number of molecules	3
	Total formula weight	1885.5
All*	Total formula weight	129816.6

*Water molecules are not included.