6A96
Cryo-EM structure of the human alpha5beta3 GABAA receptor in complex with GABA and Nb25
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (K, L, O) | Nb25 | polymer | 125 | 13673.0 | 3 | Lama glama | ||
| 2 | D, F, G, H (B, E, C, D) | Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 366 | 42052.7 | 4 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | GABA(A) receptor subunit beta-3,GABA(A) receptor subunit beta-3 |
| 3 | E (A) | Gamma-aminobutyric acid receptor subunit alpha-5,Gamma-aminobutyric acid receptor subunit alpha-5 | polymer | 392 | 44456.1 | 1 | UniProt (P31644) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | GABA(A) receptor subunit alpha-5,GABA(A) receptor subunit alpha-5 |
| 4 | I, M, O, Q (F, J, N, Q) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 4 | In PDB GlyTouCan (G09724ZC) | |||
| 5 | J, N, P, R (G, M, P, R) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 4 | In PDB GlyTouCan (G15407YE) | |||
| 6 | K (H) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 7 | L (I) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 8 | S (B) | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 1 | Chemie (ABU) |
Sequence modifications
B, E, C, D: -24 - 307 (UniProt: P28472)
B, E, C, D: 422 - 448 (UniProt: P28472)
A: -30 - 315 (UniProt: P31644)
A: 393 - 431 (UniProt: P31644)
B, E, C, D: 422 - 448 (UniProt: P28472)
| PDB | External Database | Details |
|---|---|---|
| Ser 308 | - | linker |
| Gln 309 | - | linker |
| Pro 310 | - | linker |
| Ala 311 | - | linker |
| Arg 312 | - | linker |
| Ala 313 | - | linker |
| Ala 314 | - | linker |
A: 393 - 431 (UniProt: P31644)
| PDB | External Database | Details |
|---|---|---|
| Ser 316 | - | linker |
| Gln 317 | - | linker |
| Pro 318 | - | linker |
| Ala 319 | - | linker |
| Arg 320 | - | linker |
| Ala 321 | - | linker |
| Ala 322 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 253686.0 | |
| Branched | Number of molecules | 10 |
| Total formula weight | 7973.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 103.1 | |
| All* | Total formula weight | 261762.4 |
